RecombinantRabMAb

Recombinant Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19725] to Ornithine Carbamoyltransferase/OTC
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725]
    See all Ornithine Carbamoyltransferase/OTC primary antibodies

  • Description

    Rabbit monoclonal [EPR19725] to Ornithine Carbamoyltransferase/OTC

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Ornithine Carbamoyltransferase/OTC aa 50-150. The exact sequence is proprietary.
    Database link: P00480

  • Positive control

    • WB: Human fetal liver and small intestine lysates; Mouse and rat liver lysates. IHC-P: Human liver and liver cancer tissues. IHC-Fr: Mouse liver tissue. IP: Human fetal liver lysate.

  • General notes

    Previously labelled as Ornithine Carbamoyltransferase. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab203859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P is recommended for human only.
IHC-Fr 1/500.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).
IP 1/40.

Target

  • Tissue specificity

    Mainly expressed in liver and intestinal mucosa.

  • Pathway

    Nitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1.

  • Involvement in disease

    Ornithine carbamoyltransferase deficiency

  • Sequence similarities

    Belongs to the ATCase/OTCase family.

  • Post-translational
    modifications

    Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EC 2.1.3.3 antibody
    • MGC129967 antibody
    • MGC129968 antibody
    • MGC138856 antibody
    • OCTD antibody
    • Ornithine Carbamoyltransferase antibody
    • Ornithine carbamoyltransferase mitochondrial antibody
    • Ornithine carbamoyltransferase, mitochondrial antibody
    • Ornithine transcarbamylase antibody
    • Otc antibody
    • OTC_HUMAN antibody
    • OTCase antibody
    see all

Images

  • Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Lane 1 : Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859) at 1/5000 dilution
    Lane 2 : Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human small intestine lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized Mouse liver tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green).

    Cytoplasmic staining on mouse liver is observed.

    Nuclear counterstain: DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on Human liver tissue is observed [PMID: 17893704].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    All lanes : Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859) at 1/1000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 30 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

    Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on Human liver cancer tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    No staining on Human kidney is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized Mouse kidney tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077)  at 1/1000 dilution (green).

    Weakly staining on mouse kidney.

    Nuclear counterstain: DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

  • Immunoprecipitation - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Immunoprecipitation - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

    Ornithine Carbamoyltransferase/OTC was immunoprecipitated from 0.35 mg of Human fetal liver lysate with ab203859 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab203859 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human fetal liver lysate, 10µg (Input).

    Lane 2: ab203859 IP in Human fetal liver lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab203859 in Human fetal liver lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

References (2)

Publishing research using ab203859? Please let us know so that we can cite the reference in this datasheet.

ab203859 has been referenced in 2 publications.

  • Chow JPH  et al. A modified arginine-depleting enzyme NEI-01 inhibits growth of pancreatic cancer cells. PLoS One 15:e0231633 (2020). PubMed: 32353864
  • Katrekar D  et al. In vivo RNA editing of point mutations via RNA-guided adenosine deaminases. Nat Methods 16:239-242 (2019). PubMed: 30737497

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