Recombinant
RabMAb

Recombinant Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)

Overview

  • Product name

    Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free
    See all Ornithine Carbamoyltransferase/OTC primary antibodies
  • Description

    Rabbit monoclonal [EPR19725] to Ornithine Carbamoyltransferase/OTC - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    aa 50-150. The exact sequence is proprietary.
    Database link: P00480

  • Positive control

    • WB: Human fetal liver and small intestine lysates; Mouse and rat liver lysates. IHC-P: Human liver and liver cancer tissues. IHC-Fr: Mouse liver tissue. IP: Human fetal liver lysate.
  • General notes

    ab222399 is the carrier-free version of ab203859 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab222399 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Ornithine Carbamoyltransferase.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab222399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

WB Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P is recommended for human only.

IP Use at an assay dependent concentration.

Target

  • Tissue specificity

    Mainly expressed in liver and intestinal mucosa.
  • Pathway

    Nitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1.
  • Involvement in disease

    Ornithine carbamoyltransferase deficiency
  • Sequence similarities

    Belongs to the ATCase/OTCase family.
  • Post-translational
    modifications

    Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 2.1.3.3 antibody
    • MGC129967 antibody
    • MGC129968 antibody
    • MGC138856 antibody
    • OCTD antibody
    • Ornithine Carbamoyltransferase antibody
    • Ornithine carbamoyltransferase mitochondrial antibody
    • Ornithine carbamoyltransferase, mitochondrial antibody
    • Ornithine transcarbamylase antibody
    • Otc antibody
    • OTC_HUMAN antibody
    • OTCase antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on Human liver tissue is observed [PMID: 17893704].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203859).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on Human liver cancer tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203859).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    No staining on Human kidney is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203859).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized Mouse liver tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green).

    Cytoplasmic staining on mouse liver is observed.

    Nuclear counterstain: DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203859).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized Mouse kidney tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077)  at 1/1000 dilution (green).

    Weakly staining on mouse kidney.

    Nuclear counterstain: DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203859).

  • Ornithine Carbamoyltransferase/OTC Western blot was performed from the immunoprecipitate using ab203859 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human fetal liver lysate, 10µg (Input).

    Lane 2: ab203859 IP in Human fetal liver lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab203859 in Human fetal liver lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203859).

References

ab222399 has not yet been referenced specifically in any publications.

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