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I am using this antibody in WB and obtaining many background bands and no band around the predicted molecular weight:
Sample -human cancer cell line; loaded 100ug total protein
block - 5% milk
1' ab - 1:500, 1:1000
2' ab - 1:6000
Asked on Apr 25 2012
Thank you for contacting Abcam regarding ab66067.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.
Answered on Apr 25 2012