Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (ab220205)
Key features and details
- Mouse monoclonal [ODC1/487] to Ornithine Decarboxylase/ODC
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487]
See all Ornithine Decarboxylase/ODC primary antibodies -
Description
Mouse monoclonal [ODC1/487] to Ornithine Decarboxylase/ODC -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Ornithine Decarboxylase.
Database link: P11926 -
Positive control
- IHC-P: Rat pancreas tissue; Mouse pancreas and small intestine tissue. ICC: LNCap cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab220205 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
ODC1/487 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 1 - 2 µg/ml.
|
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT). |
Notes |
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ICC/IF
Use a concentration of 1 - 2 µg/ml. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT). |
Target
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Pathway
Amine and polyamine biosynthesis; putrescine biosynthesis via L-ornithine pathway; putrescine from L-ornithine: step 1/1. -
Sequence similarities
Belongs to the Orn/Lys/Arg decarboxylase class-II family. -
Post-translational
modificationsS-Nitrosylation inhibits the enzyme. S-Nitrosylated in vitro on 4 cysteine residues. - Information by UniProt
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Database links
- Entrez Gene: 4953 Human
- Entrez Gene: 18263 Mouse
- Entrez Gene: 24609 Rat
- Omim: 165640 Human
- SwissProt: P11926 Human
- SwissProt: P00860 Mouse
- SwissProt: P09057 Rat
- Unigene: 467701 Human
see all -
Alternative names
- DCOR_HUMAN antibody
- Dodc1 antibody
- Odc 1 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat pancreas tissue labeling Ornithine Decarboxylase with ab220205 at 1 µg/ml.
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Immunofluorescence staining of LNCap (Human prostate cancer cell line) cells labeling Ornithine Decarboxylase/ODC (Green) using ab220205 at 2 µg/ml. The counterstain was DAPI (Blue).
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse pancreas tissue labeling Ornithine Decarboxylase with ab220205 at 1 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue labeling Ornithine Decarboxylase with ab220205 at 1 µg/ml.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab220205 has not yet been referenced specifically in any publications.