Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (ab220205)
Key features and details
- Mouse monoclonal [ODC1/487] to Ornithine Decarboxylase/ODC
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG2a
Overview
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Product name
Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487]
See all Ornithine Decarboxylase/ODC primary antibodies -
Description
Mouse monoclonal [ODC1/487] to Ornithine Decarboxylase/ODC -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human -
Immunogen
Recombinant full length protein corresponding to Human Ornithine Decarboxylase.
Database link: P11926 -
Positive control
- IHC-P: Rat pancreas tissue; Mouse pancreas and small intestine tissue. ICC: LNCap cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab220205 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
ODC1/487 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab220205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | Use a concentration of 1 - 2 µg/ml. | |
IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT). |
Target
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Pathway
Amine and polyamine biosynthesis; putrescine biosynthesis via L-ornithine pathway; putrescine from L-ornithine: step 1/1. -
Sequence similarities
Belongs to the Orn/Lys/Arg decarboxylase class-II family. -
Post-translational
modificationsS-Nitrosylation inhibits the enzyme. S-Nitrosylated in vitro on 4 cysteine residues. - Information by UniProt
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Database links
- Entrez Gene: 4953 Human
- Entrez Gene: 18263 Mouse
- Entrez Gene: 24609 Rat
- Omim: 165640 Human
- SwissProt: P11926 Human
- SwissProt: P00860 Mouse
- SwissProt: P09057 Rat
- Unigene: 467701 Human
see all -
Alternative names
- DCOR_HUMAN antibody
- Dodc1 antibody
- Odc 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase antibody [ODC1/487] (ab220205)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat pancreas tissue labeling Ornithine Decarboxylase with ab220205 at 1 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (ab220205)
Immunofluorescence staining of LNCap (Human prostate cancer cell line) cells labeling Ornithine Decarboxylase/ODC (Green) using ab220205 at 2 µg/ml. The counterstain was DAPI (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (ab220205)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse pancreas tissue labeling Ornithine Decarboxylase with ab220205 at 1 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (ab220205)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue labeling Ornithine Decarboxylase with ab220205 at 1 µg/ml.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab220205 has not yet been referenced specifically in any publications.