Product nameAnti-ORP-3 antibody
See all ORP-3 primary antibodies
DescriptionRabbit polyclonal to ORP-3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
Previously labelled as OSBPL3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192998 was affinity purified using an epitope specific to ORP-3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab192998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 101 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Tissue specificityIsoform 1a, isoform 1b, isoform 1c and isoform 1d are highly expressed in brain, bone marrow, colon, kidney, lung, skeletal muscle, spleen, thymus and thyroid. Not expressed in heart and liver. Isoform 2a, isoform 2b, isoform 2c and isoform 2d are expressed in brain, bone marrow, kidney, skeletal muscle, spleen, thymus and thyroid. Not expressed in heart, liver and lung.
Sequence similaritiesBelongs to the OSBP family.
Contains 1 PH domain.
- Information by UniProt
- DKFZp667P1518 antibody
- KIAA0704 antibody
- MGC21526 antibody
All lanes : Anti-ORP-3 antibody (ab192998) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 101 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
Detection of ORP-3 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab192998 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab192998 has not yet been referenced specifically in any publications.