Overview

  • Product name

  • Description

    Rabbit polyclonal to ORP-3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human ORP-3 aa 456-506. The exact sequence is proprietary. (NP_056365.1).
    Sequence:

    EVLLSPSSSENEISDDDSYVSDISDNLSLDNLSNDLDNERQTLGPVLDSG R


    Database link: Q9H4L5

  • Positive control

    • HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
  • General notes

    Previously labelled as OSBPL3. 

Properties

Applications

Our Abpromise guarantee covers the use of ab192998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 101 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Tissue specificity

    Isoform 1a, isoform 1b, isoform 1c and isoform 1d are highly expressed in brain, bone marrow, colon, kidney, lung, skeletal muscle, spleen, thymus and thyroid. Not expressed in heart and liver. Isoform 2a, isoform 2b, isoform 2c and isoform 2d are expressed in brain, bone marrow, kidney, skeletal muscle, spleen, thymus and thyroid. Not expressed in heart, liver and lung.
  • Sequence similarities

    Belongs to the OSBP family.
    Contains 1 PH domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp667P1518 antibody
    • KIAA0704 antibody
    • MGC21526 antibody
    • ORP 3 antibody
    • ORP-3 antibody
    • ORP3 antibody
    • OSBL3_HUMAN antibody
    • OSBP related protein 3 antibody
    • OSBP-related protein 3 antibody
    • OSBP3 antibody
    • Osbpl3 antibody
    • Oxysterol binding protein 3 antibody
    • Oxysterol binding protein like 3 antibody
    • Oxysterol binding protein related protein 3 antibody
    • Oxysterol-binding protein-related protein 3 antibody
    see all

Images

  • All lanes : Anti-ORP-3 antibody (ab192998) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 101 kDa


    Exposure time: 3 minutes


    Lysates prepared using NETN lysis buffer.

  • Detection of ORP-3 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab192998 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (lane 1). Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab192998 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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