Overview

  • Product name

    Anti-ORP1 antibody [EPR8646]
    See all ORP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8646] to ORP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human ORP1 aa 900-1000.

  • Positive control

    • WB: A549, 293T, U87-MG cell lysates; Mouse brain and heart lysates; Rat brain lysates FC: A549 cells ICC/FC: A549 and U87-MG cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8646
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/1000 - 1/10000. Detects a band of approximately 50-108 kDa (predicted molecular weight: 108 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • All lanes : Anti-ORP1 antibody [EPR8646] (ab131165) at 1/10000 dilution (Purified)

      Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
      Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
      Lane 3 : Mouse brain lysates
      Lane 4 : Mouse heart lysates
      Lane 5 : Rat brain lysates
      Lane 6 : Rat heart lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 108 kDa
      Observed band size: 108,50 kDa
      why is the actual band size different from the predicted?



      Two mRNA variants produce a 108 kDa long ORP1 (ORP1L) and a 50 KDa short ORP1 (ORP1S) separately, which is consistent with what has been described in PMID: 12631712.

    • Anti-ORP1 antibody [EPR8646] (ab131165) at 1/1000 dilution + 293T (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 108 kDa
      Observed band size: 108,50 kDa why is the actual band size different from the predicted?



      Two mRNA variants produce a 108 kDa long ORP1 (ORP1L) and a 50 KDa short ORP1 (ORP1S) separately, which is consistent with what has been described in PMID: 12631712.

    • Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1/60 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1:200 dilution (2.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • All lanes : Anti-ORP1 antibody [EPR8646] (ab131165) at 1/1000 dilution (unpurified)

      Lane 1 : A549 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 108 kDa
      Observed band size: 50-108 kDa why is the actual band size different from the predicted?

    • ab131165 (unpurified) staining ORP1 in U87-MG (human glioblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

      DAPI was used as a nuclear counterstain and the negative control was PBS only.

    • ab131165 (unpurified) at 1/10 dilution staining ORP1 in permeabilized A549 cells by Flow cytometry (red). Rabbit IgG negative control (green).

    References

    This product has been referenced in:

    • Dong J  et al. Allosteric enhancement of ORP1-mediated cholesterol transport by PI(4,5)P2/PI(3,4)P2. Nat Commun 10:829 (2019). Read more (PubMed: 30783101) »
    • Atakpa P  et al. IP3 Receptors Preferentially Associate with ER-Lysosome Contact Sites and Selectively Deliver Ca2+ to Lysosomes. Cell Rep 25:3180-3193.e7 (2018). Read more (PubMed: 30540949) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131165.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up