Product nameAnti-ORP150 antibody [EP5891]
See all ORP150 primary antibodies
DescriptionRabbit monoclonal [EP5891] to ORP150
Tested applicationsSuitable for: IP, ICC/IF, WB, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ORP150 aa 950-1050 (C terminal). The exact sequence is proprietary.
- Human colon and liver tissues; MCF7 cells; 293T, HeLa, HepG2, MCF7 and SH-SY5Y cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|ICC/IF||1/250 - 1/500.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 111 kDa.|
|Flow Cyt||Use at an assay dependent concentration.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionHas a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
Tissue specificityHighly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.
Sequence similaritiesBelongs to the heat shock protein 70 family.
Cellular localizationEndoplasmic reticulum lumen.
- Information by UniProt
- 150 kDa oxygen regulated protein antibody
- 150 kDa oxygen-regulated protein antibody
- 170 kDa glucose regulated protein antibody
All lanes : Anti-ORP150 antibody [EP5891] (ab134944) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 111 kDa
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human colon tissue, labelling ORP150 using ab134944 at a 1/250 dilution.
Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling ORP150 with unpurified ab134944 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human liver tissue, labelling ORP150 using ab134944 at a 1/250 dilution.
Immunofluorescence staining of MCF7 cells, labelling ORP150 using ab134944 at a 1/250 dilution (red). Cell nuclei are shown in blue.
This product has been referenced in:
- Kuljittichanok D et al. Effect of Derris scandens extract on a human hepatocellular carcinoma cell line. Oncol Lett 16:1943-1952 (2018). Read more (PubMed: 30034552) »
- Giffin L et al. Modulation of Kaposi's sarcoma-associated herpesvirus interleukin-6 function by hypoxia-upregulated protein 1. J Virol 88:9429-41 (2014). Read more (PubMed: 24920810) »