Anti-ORP150 antibody [EP5891] (ab134944)
Key features and details
- Rabbit monoclonal [EP5891] to ORP150
- Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ORP150 antibody [EP5891]
See all ORP150 primary antibodies -
Description
Rabbit monoclonal [EP5891] to ORP150 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human ORP150 aa 950-1050 (C terminal). The exact sequence is proprietary.
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Positive control
- Human colon and liver tissues; MCF7 cells; 293T, HeLa, HepG2, MCF7 and SH-SY5Y cell lysates IP: HeLa whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP5891 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IP |
1/10 - 1/100.
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ICC/IF |
1/250 - 1/500.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 111 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
IP
1/10 - 1/100. |
ICC/IF
1/250 - 1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 111 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. -
Tissue specificity
Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 10525 Human
- Omim: 601746 Human
- SwissProt: Q9Y4L1 Human
- Unigene: 277704 Human
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Alternative names
- 150 kDa oxygen regulated protein antibody
- 150 kDa oxygen-regulated protein antibody
- 170 kDa glucose regulated protein antibody
see all
Images
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All lanes : Anti-ORP150 antibody [EP5891] (ab134944) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 111 kDa -
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human colon tissue, labelling ORP150 using ab134944 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling ORP150 with unpurified ab134944 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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purifiedab134944 at 1/50 dilution (2µg) immunoprecipitating ORP150 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab134944 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134944 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/2000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 150 kDa -
Immunofluorescence staining of MCF7 cells, labelling ORP150 using ab134944 at a 1/250 dilution (red). Cell nuclei are shown in blue.
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Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human liver tissue, labelling ORP150 using ab134944 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab134944 has been referenced in 5 publications.
- Liu J & Wang Y Long non-coding RNA KCNQ1OT1 facilitates the progression of cervical cancer and tumor growth through modulating miR-296-5p/HYOU1 axis. Bioengineered 12:8753-8767 (2021). PubMed: 34704918
- Kuljittichanok D et al. Effect of Derris scandens extract on a human hepatocellular carcinoma cell line. Oncol Lett 16:1943-1952 (2018). PubMed: 30034552
- Abu El-Asrar AM et al. Association of 150-kDa oxygen-regulated protein with vascular endothelial growth factor in proliferative diabetic retinopathy. Acta Ophthalmol 96:e460-e467 (2018). PubMed: 29098793
- Giffin L et al. Modulation of Kaposi's sarcoma-associated herpesvirus interleukin-6 function by hypoxia-upregulated protein 1. J Virol 88:9429-41 (2014). PubMed: 24920810
- Cui GQ et al. Proteomic analysis of meningiomas. Acta Neurol Belg 114:187-94 (2014). WB ; Human . PubMed: 24085542