Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5890] to ORP150
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-ORP150 antibody [EPR5890]
See all ORP150 primary antibodies
DescriptionRabbit monoclonal [EPR5890] to ORP150
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ORP150 aa 50-150. The exact sequence is proprietary.
(Peptide available as
- Human fetal liver, Rat liver, Rat pancreas, 293T, MCF7, MDA-MB-435S, SH-SY5Y and HeLa lysates; Human liver tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.40 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab124884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 111 kDa).Can be blocked with ORP150 peptide (ab156891).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionHas a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
Tissue specificityHighly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.
Sequence similaritiesBelongs to the heat shock protein 70 family.
Cellular localizationEndoplasmic reticulum lumen.
- Information by UniProt
- 150 kDa oxygen regulated protein antibody
- 150 kDa oxygen-regulated protein antibody
- 170 kDa glucose regulated protein antibody
All lanes : Anti-ORP150 antibody [EPR5890] (ab124884) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Rat liver lysate
Lane 3 : Rat pancreas lysate
Lane 4 : 293T lysate
Lane 5 : MCF7 lysate
Lane 6 : MDA-MB-435S lysate
Lane 7 : SH-SY5Y lysate
Lane 8 : HeLa lysate
Lysates/proteins at 10 µg/ml per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 111 kDa
Overlay histogram showing HepG2 cells stained with ab124884 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124884, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab124884, at 1/250 dilution, staining ORP150 in paraffin-embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124884 has been referenced in 3 publications.
- Balboa D et al. Insulin mutations impair beta-cell development in a patient-derived iPSC model of neonatal diabetes. Elife 7:N/A (2018). PubMed: 30412052
- Wang ZS et al. Astragaloside IV attenuates proteinuria in streptozotocin-induced diabetic nephropathy via the inhibition of endoplasmic reticulum stress. BMC Nephrol 16:44 (2015). PubMed: 25886386
- Chiu HC et al. High-throughput quantitative proteomic analysis of dengue virus type 2 infected A549 cells. PLoS One 9:e93305 (2014). WB ; Human . PubMed: 24671231