Key features and details
- Rabbit polyclonal to OS9
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-OS9 antibody
See all OS9 primary antibodies
DescriptionRabbit polyclonal to OS9
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human OS9 aa 232-413.
Database link: Q13438
- IHC-P: Human testis tissue and lung cancer tissues. WB: HepG2 and HEK-293 whole cell lysates. ICC/IF: HeLa cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab225915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).|
|ICC/IF||1/50 - 1/200.|
FunctionLectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
Tissue specificityUbiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
Sequence similaritiesBelongs to the OS-9 family.
Contains 1 PRKCSH domain.
modificationsIntramolecular disulfide bonds.
Isoform 1 and isoform 2 are N-glycosylated.
Cellular localizationEndoplasmic reticulum lumen.
- Information by UniProt
- Amplified in osteosarcoma 9 antibody
- amplified in osteosarcoma antibody
- ERLEC2 antibody
All lanes : Anti-OS9 antibody (ab225915) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for OS9 (green) using ab225915 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human testis tissue stained for OS9 using ab225915 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human lung cancer tissue stained for OS9 using ab225915 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225915 has not yet been referenced specifically in any publications.