Overview

  • Product name

  • Description

    Rabbit polyclonal to OS9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human OS9 aa 232-413.
    Database link: Q13438

  • Positive control

    • IHC-P: Human testis tissue and lung cancer tissues. WB: HepG2 and HEK-293 whole cell lysates. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab225915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/500 - 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
ICC/IF 1/50 - 1/200.

Target

  • Function

    Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
  • Tissue specificity

    Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
  • Sequence similarities

    Belongs to the OS-9 family.
    Contains 1 PRKCSH domain.
  • Post-translational
    modifications

    Intramolecular disulfide bonds.
    Isoform 1 and isoform 2 are N-glycosylated.
  • Cellular localization

    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • Amplified in osteosarcoma 9 antibody
    • amplified in osteosarcoma antibody
    • ERLEC2 antibody
    • OS-9 antibody
    • Os9 antibody
    • OS9_HUMAN antibody
    • Osteosarcoma amplified 9 endoplasmic reticulum lectin antibody
    • Protein OS-9 antibody
    see all

Images

  • All lanes : Anti-OS9 antibody (ab225915) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 76 kDa
    Observed band size: 76 kDa

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for OS9 (green) using ab225915 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin-embedded human testis tissue stained for OS9 using ab225915 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human lung cancer tissue stained for OS9 using ab225915 at 1/100 dilution in immunohistochemical analysis.

References

ab225915 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab225915.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up