Product nameAnti-OS9 antibody [EPR4272(2)]
See all OS9 primary antibodies
DescriptionRabbit monoclonal [EPR4272(2)] to OS9
Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human OS9 aa 600-700 (C terminal). The exact sequence is proprietary.
- NIH3T3, 293T, HL-60, and HeLa cell lysates, Mouse testis tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 76 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
FunctionLectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
Tissue specificityUbiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
Sequence similaritiesBelongs to the OS-9 family.
Contains 1 PRKCSH domain.
modificationsIntramolecular disulfide bonds.
Isoform 1 and isoform 2 are N-glycosylated.
Cellular localizationEndoplasmic reticulum lumen.
- Information by UniProt
- Amplified in osteosarcoma 9 antibody
- amplified in osteosarcoma antibody
- ERLEC2 antibody
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using ab109510 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-OS9 antibody [EPR4272(2)] (ab109510) at 1/10000 dilution
Lane 1 : NIH3T3 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HL-60 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 76 kDa
This product has been referenced in:
- Sun S et al. IRE1a is an endogenous substrate of endoplasmic-reticulum-associated degradation. Nat Cell Biol 17:1546-55 (2015). WB, IHC . Read more (PubMed: 26551274) »
- Sun S et al. Sel1L is indispensable for mammalian endoplasmic reticulum-associated degradation, endoplasmic reticulum homeostasis, and survival. Proc Natl Acad Sci U S A 111:E582-91 (2014). Read more (PubMed: 24453213) »