• Product name

    Anti-OS9 antibody [EPR4272(2)]
    See all OS9 primary antibodies
  • Description

    Rabbit monoclonal [EPR4272(2)] to OS9
  • Host species

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human OS9 aa 600-700 (C terminal). The exact sequence is proprietary.

  • Positive control

    • NIH3T3, 293T, HL-60, and HeLa cell lysates, Mouse testis tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab109510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 76 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
    • Tissue specificity

      Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
    • Sequence similarities

      Belongs to the OS-9 family.
      Contains 1 PRKCSH domain.
    • Post-translational

      Intramolecular disulfide bonds.
      Isoform 1 and isoform 2 are N-glycosylated.
    • Cellular localization

      Endoplasmic reticulum lumen.
    • Information by UniProt
    • Database links

    • Alternative names

      • Amplified in osteosarcoma 9 antibody
      • amplified in osteosarcoma antibody
      • ERLEC2 antibody
      • OS-9 antibody
      • Os9 antibody
      • OS9_HUMAN antibody
      • Osteosarcoma amplified 9 endoplasmic reticulum lectin antibody
      • Protein OS-9 antibody
      see all


    • Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using ab109510 at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-OS9 antibody [EPR4272(2)] (ab109510) at 1/10000 dilution

      Lane 1 : NIH3T3 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HL-60 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 76 kDa


    This product has been referenced in:

    • Sun S  et al. IRE1a is an endogenous substrate of endoplasmic-reticulum-associated degradation. Nat Cell Biol 17:1546-55 (2015). WB, IHC . Read more (PubMed: 26551274) »
    • Sun S  et al. Sel1L is indispensable for mammalian endoplasmic reticulum-associated degradation, endoplasmic reticulum homeostasis, and survival. Proc Natl Acad Sci U S A 111:E582-91 (2014). Read more (PubMed: 24453213) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8% Bis-Tris gel, MOPS buffer)
    Human Cell lysate - whole cell (HEK293T)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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    Submitted Mar 02 2015

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