Recombinant Anti-OS9 antibody [EPR4272(2)] (ab109510)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4272(2)] to OS9
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-OS9 antibody [EPR4272(2)]
See all OS9 primary antibodies -
Description
Rabbit monoclonal [EPR4272(2)] to OS9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- NIH3T3, 293T, HL-60, and HeLa cell lysates, Mouse testis tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4272(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 76 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 76 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4. -
Tissue specificity
Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined. -
Sequence similarities
Belongs to the OS-9 family.
Contains 1 PRKCSH domain. -
Post-translational
modificationsIntramolecular disulfide bonds.
Isoform 1 and isoform 2 are N-glycosylated. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 10956 Human
- Entrez Gene: 216440 Mouse
- Entrez Gene: 362891 Rat
- Omim: 609677 Human
- SwissProt: Q13438 Human
- SwissProt: Q8K2C7 Mouse
- SwissProt: Q5RKH6 Rat
- Unigene: 527861 Human
see all -
Alternative names
- Amplified in osteosarcoma 9 antibody
- amplified in osteosarcoma antibody
- ERLEC2 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using ab109510 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-OS9 antibody [EPR4272(2)] (ab109510) at 1/10000 dilution
Lane 1 : NIH3T3 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HL-60 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 76 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab109510 has been referenced in 7 publications.
- Sargin P et al. Lactiplantibacillus plantarum 299v supplementation modulates β-cell ER stress and antioxidative defense pathways and prevents type 1 diabetes in gluten-free BioBreeding rats. Gut Microbes 14:2136467 (2022). PubMed: 36261888
- Di XJ et al. Proteostasis Regulators Restore Function of Epilepsy-Associated GABAA Receptors. Cell Chem Biol 28:46-59.e7 (2021). PubMed: 32888501
- Galeone A et al. Regulation of BMP4/Dpp retrotranslocation and signaling by deglycosylation. Elife 9:N/A (2020). PubMed: 32720893
- Brody MJ et al. Disruption of valosin-containing protein activity causes cardiomyopathy and reveals pleiotropic functions in cardiac homeostasis. J Biol Chem 294:8918-8929 (2019). PubMed: 31006653
- Bhattacharya A et al. Hepatic Sel1L-Hrd1 ER-associated degradation (ERAD) manages FGF21 levels and systemic metabolism via CREBH. EMBO J 37:N/A (2018). PubMed: 30389665
- Sun S et al. IRE1a is an endogenous substrate of endoplasmic-reticulum-associated degradation. Nat Cell Biol 17:1546-55 (2015). WB, IHC . PubMed: 26551274
- Sun S et al. Sel1L is indispensable for mammalian endoplasmic reticulum-associated degradation, endoplasmic reticulum homeostasis, and survival. Proc Natl Acad Sci U S A 111:E582-91 (2014). PubMed: 24453213