RecombinantRabMAb

Recombinant Anti-OS9 antibody [EPR4272(2)] (ab109510)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody [EPR4272(2)] (ab109510)
  • Western blot - Anti-OS9 antibody [EPR4272(2)] (ab109510)
  • Anti-OS9 antibody [EPR4272(2)] (ab109510)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4272(2)] to OS9
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-OS9 antibody [EPR4272(2)]
    See all OS9 primary antibodies

  • Description

    Rabbit monoclonal [EPR4272(2)] to OS9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human OS9 aa 600-700 (C terminal). The exact sequence is proprietary.

  • Positive control

    • NIH3T3, 293T, HL-60, and HeLa cell lysates, Mouse testis tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab109510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 76 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.

    • Tissue specificity

      Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.

    • Sequence similarities

      Belongs to the OS-9 family.
      Contains 1 PRKCSH domain.

    • Post-translational
      modifications

      Intramolecular disulfide bonds.
      Isoform 1 and isoform 2 are N-glycosylated.

    • Cellular localization

      Endoplasmic reticulum lumen.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Amplified in osteosarcoma 9 antibody
      • amplified in osteosarcoma antibody
      • ERLEC2 antibody
      • OS-9 antibody
      • Os9 antibody
      • OS9_HUMAN antibody
      • Osteosarcoma amplified 9 endoplasmic reticulum lectin antibody
      • Protein OS-9 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody [EPR4272(2)] (ab109510)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody [EPR4272(2)] (ab109510)

      Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using ab109510 at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-OS9 antibody [EPR4272(2)] (ab109510)
      Western blot - Anti-OS9 antibody [EPR4272(2)] (ab109510)
      All lanes : Anti-OS9 antibody [EPR4272(2)] (ab109510) at 1/10000 dilution

      Lane 1 : NIH3T3 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HL-60 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 76 kDa

    • Anti-OS9 antibody [EPR4272(2)] (ab109510)
      Anti-OS9 antibody [EPR4272(2)] (ab109510)

    References (4)

    Publishing research using ab109510? Please let us know so that we can cite the reference in this datasheet.

    ab109510 has been referenced in 4 publications.

    • Galeone A  et al. Regulation of BMP4/Dpp retrotranslocation and signaling by deglycosylation. Elife 9:N/A (2020). PubMed: 32720893
    • Bhattacharya A  et al. Hepatic Sel1L-Hrd1 ER-associated degradation (ERAD) manages FGF21 levels and systemic metabolism via CREBH. EMBO J 37:N/A (2018). PubMed: 30389665
    • Sun S  et al. IRE1a is an endogenous substrate of endoplasmic-reticulum-associated degradation. Nat Cell Biol 17:1546-55 (2015). WB, IHC . PubMed: 26551274
    • Sun S  et al. Sel1L is indispensable for mammalian endoplasmic reticulum-associated degradation, endoplasmic reticulum homeostasis, and survival. Proc Natl Acad Sci U S A 111:E582-91 (2014). PubMed: 24453213

    Customer reviews and Q&As

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    Western blot abreview for Anti-OS9 antibody [EPR4272(2)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8% Bis-Tris gel, MOPS buffer)
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Specification
    HEK293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    Verified customer

    Submitted Mar 02 2015

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