Anti-OSBP1 antibody (ab126182)

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Western blot - Anti-OSBP1 antibody (ab126182)

    Key features and details

    • Rabbit polyclonal to OSBP1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-OSBP1 antibody
      See all OSBP1 primary antibodies

    • Description

      Rabbit polyclonal to OSBP1

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Rabbit

    • Immunogen

      Recombinant fragment, corresponding to a region within amino acids 571-746 of Human OSBP1 (P22059).

    • Positive control

      • Jurkat, Raji and K562 whole cell lysates.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab126182 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 89 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 89 kDa.

    Target

    • Function

      Binds cholesterol and a range of oxysterols. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylate ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Belongs to the OSBP family.
      Contains 1 PH domain.

    • Domain

      The PH and Ala/Gly-rich domains control cholesterol binding without affecting 25-hydroxycholesterol binding.
      The second coiled-coil domain is required for interaction with the tyrosine phosphatase.

    • Cellular localization

      Cytoplasm. Golgi apparatus membrane. When bound to oxysterols, translocates to the periphery of Golgi membranes.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • OSBP 1 antibody
      • OSBP antibody
      • OSBP1_HUMAN antibody
      • Oxysterol binding protein 1 antibody
      • Oxysterol-binding protein 1 antibody
      see all

    Images

    • Western blot - Anti-OSBP1 antibody (ab126182)
      Western blot - Anti-OSBP1 antibody (ab126182)
      All lanes : Anti-OSBP1 antibody (ab126182) at 1/1000 dilution

      Lane 1 : Jurkat whole cell lysate
      Lane 2 : Raji whole cell lysate
      Lane 3 : K562 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 89 kDa



      7.5% SDS PAGE

    References (0)

    Publishing research using ab126182? Please let us know so that we can cite the reference in this datasheet.

    ab126182 has not yet been referenced specifically in any publications.

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