Product nameAnti-OSBP1 antibody
See all OSBP1 primary antibodies
DescriptionRabbit polyclonal to OSBP1
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192995 was affinity purified using an epitope specific to OSBP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab192995 in the following tested applications.
|WB||1/2000 - 1/10000. Predicted molecular weight: 89 kDa.|
FunctionBinds cholesterol and a range of oxysterols. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylate ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the OSBP family.
Contains 1 PH domain.
DomainThe PH and Ala/Gly-rich domains control cholesterol binding without affecting 25-hydroxycholesterol binding.
The second coiled-coil domain is required for interaction with the tyrosine phosphatase.
Cellular localizationCytoplasm. Golgi apparatus membrane. When bound to oxysterols, translocates to the periphery of Golgi membranes.
- Information by UniProt
- OSBP 1 antibody
- OSBP antibody
- OSBP1_HUMAN antibody
All lanes : Anti-OSBP1 antibody (ab192995) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 89 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
ab192995 has not yet been referenced specifically in any publications.