Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab5915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 89.4 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionBinds cholesterol and a range of oxysterols. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylate ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the OSBP family.
Contains 1 PH domain.
DomainThe PH and Ala/Gly-rich domains control cholesterol binding without affecting 25-hydroxycholesterol binding.
The second coiled-coil domain is required for interaction with the tyrosine phosphatase.
Cellular localizationCytoplasm. Golgi apparatus membrane. When bound to oxysterols, translocates to the periphery of Golgi membranes.
- Information by UniProt
- OSBP 1 antibody
- OSBP antibody
- OSBP1_HUMAN antibody
Anti-OSBP1 antibody (ab5915) at 2 µg/ml + Lysate prepared from human Liver at 35 µg
Developed using the ECL technique.
Predicted band size: 89.4 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Yu CJ et al. ZNF307 (Zinc Finger Protein 307) Acts as a Negative Regulator of Pressure Overload-Induced Cardiac Hypertrophy. Hypertension 69:615-624 (2017). Read more (PubMed: 28223477) »
- Wang PX et al. Hepatocyte TRAF3 promotes liver steatosis and systemic insulin resistance through targeting TAK1-dependent signalling. Nat Commun 7:10592 (2016). Read more (PubMed: 26882989) »