Overview

  • Product name

    Anti-OSBP1 antibody - N-terminal
    See all OSBP1 primary antibodies
  • Description

    Rabbit polyclonal to OSBP1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Dog, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within OSBP1 aa 1-50 (N terminal). The exact sequence is proprietary. (NP_002547.1).
    Sequence:

    MAATELRGVVGPGPAAIAALGGGGAGPPVVGGGGGRGDAGPGSGAASGTV


    Database link: P22059

  • Positive control

    • 293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab192990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    Images

    • Detection of OSBP1 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab192990 at 6 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    References

    This product has been referenced in:

    • Civra A  et al. 25-Hydroxycholesterol and 27-hydroxycholesterol inhibit human rotavirus infection by sequestering viral particles into late endosomes. Redox Biol 19:318-330 (2018). Read more (PubMed: 30212801) »
    See 1 Publication for this product

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