Overview

  • Product name

  • Description

    Rabbit polyclonal to OSBPL5/ORP5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human OSBPL5/ORP5 aa 242-458 (internal sequence).

  • Positive control

    • HeLa and A549 whole cell lysate Breast cancer tissue
  • General notes

     This product was previously labelled as OSBPL5

     

Properties

Applications

Our Abpromise guarantee covers the use of ab127171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 99 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used.

Target

  • Relevance

    OSBPL5 is a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Transcript variants encoding different isoforms have been identified.
  • Database links

  • Alternative names

    • DKFZp586H1719 antibody
    • FLJ31948 antibody
    • KIAA1534 antibody
    • OBPH1 antibody
    • ORP 5 antibody
    • ORP5 antibody
    • OSBP related protein 5 antibody
    • Oxysterol binding protein homolog 1 antibody
    • Oxysterol binding protein homologue 1 antibody
    • Oxysterol binding protein like 5 antibody
    • Oxysterol binding protein related protein 5 antibody
    see all

Images

  • ab127171, at 1/500 dilution, staining OSBPL5/ORP5 in paraffin-embedded breast cancer tissue by immunohistochemistry

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling OSBPL5/ORP5 using ab127171 at 1/500 dilution (green) in ICC/IF. 

    The nuclear counter stain is Hoechst 33342 (blue).

  • Anti-OSBPL5/ORP5 antibody (ab127171) at 1/500 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 99 kDa



    7.5% SDS PAGE

References

This product has been referenced in:

  • Li J  et al. Oxysterol binding protein-related protein 8 mediates the cytotoxicity of 25-hydroxycholesterol. J Lipid Res 57:1845-1853 (2016). Read more (PubMed: 27530118) »
See 1 Publication for this product

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