Overview

  • Product name

  • Description

    Goat polyclonal to OSBPL5/ORP5
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise both reported isoforms (NP_065947.1; NP_663613.1).
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human OSBPL5/ORP5 aa 2-14 (N terminal).
    Sequence:

    KEEAFLRRRFSLC

  • Positive control

    • Human Spleen lysate.
  • General notes

     This product was previously labelled as OSBPL5

     

Properties

Applications

Our Abpromise guarantee covers the use of ab59016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).

Target

  • Relevance

    OSBPL5 is a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Transcript variants encoding different isoforms have been identified.
  • Database links

  • Alternative names

    • DKFZp586H1719 antibody
    • FLJ31948 antibody
    • KIAA1534 antibody
    • OBPH1 antibody
    • ORP 5 antibody
    • ORP5 antibody
    • OSBP related protein 5 antibody
    • Oxysterol binding protein homolog 1 antibody
    • Oxysterol binding protein homologue 1 antibody
    • Oxysterol binding protein like 5 antibody
    • Oxysterol binding protein related protein 5 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human kidney staining OSBPL5/ORP5 with ab59016 at 5 μg/ml

  • Anti-OSBPL5/ORP5 antibody (ab59016) at 2 µg/ml + Human Spleen lysate (35µg protein in RIPA buffer)

    Predicted band size: 99 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human liver staining OSBPL5/ORP5 with ab59016 at 5 μg/ml

References

This product has been referenced in:

  • Sohn M  et al. PI(4,5)P2 controls plasma membrane PI4P and PS levels via ORP5/8 recruitment to ER-PM contact sites. J Cell Biol 217:1797-1813 (2018). Read more (PubMed: 29472386) »
  • Ghai R  et al. ORP5 and ORP8 bind phosphatidylinositol-4, 5-biphosphate (PtdIns(4,5)P2) and regulate its level at the plasma membrane. Nat Commun 8:757 (2017). Read more (PubMed: 28970484) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - Methanol, Triton X-100 or Saponin
Specification
HeLa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 10 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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