Key features and details
- Goat polyclonal to OSBPL5/ORP5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-OSBPL5/ORP5 antibody
See all OSBPL5/ORP5 primary antibodies
DescriptionGoat polyclonal to OSBPL5/ORP5
SpecificityThis antibody is expected to recognise both reported isoforms (NP_065947.1; NP_663613.1).
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- Human Spleen lysate.
This product was previously labelled as OSBPL5
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab59016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
1 hour primary incubation is recommended for this product.
RelevanceOSBPL5 is a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Transcript variants encoding different isoforms have been identified.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human kidney staining OSBPL5/ORP5 with ab59016 at 5 μg/ml
Anti-OSBPL5/ORP5 antibody (ab59016) at 2 µg/ml + Human Spleen lysate (35µg protein in RIPA buffer)
Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human liver staining OSBPL5/ORP5 with ab59016 at 5 μg/ml
ab59016 has been referenced in 3 publications.
- Sohn M et al. PI(4,5)P2 controls plasma membrane PI4P and PS levels via ORP5/8 recruitment to ER-PM contact sites. J Cell Biol 217:1797-1813 (2018). PubMed: 29472386
- Du X et al. Oxysterol-binding protein-related protein 5 (ORP5) promotes cell proliferation by activation of mTORC1 signaling. J Biol Chem 293:3806-3818 (2018). PubMed: 29358326
- Ghai R et al. ORP5 and ORP8 bind phosphatidylinositol-4, 5-biphosphate (PtdIns(4,5)P2) and regulate its level at the plasma membrane. Nat Commun 8:757 (2017). PubMed: 28970484