Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9333] to OSBPL9
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-OSBPL9 antibody [EPR9333]
See all OSBPL9 primary antibodies
DescriptionRabbit monoclonal [EPR9333] to OSBPL9
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human OSBPL9 aa 650-750. The exact sequence is proprietary.
- Human fetal brain, Jurkat, 293T and HepG2 cell lysates; Human kidney tissue; 293T cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab151691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 83 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceThe OSBPL9 gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. OSBPL9 appears to be in a distinct OSBP subfamily and shares relatively little homology in the sterol-binding domain with other OSBPs. Multiple transcript variants have been identified, most of which encode distinct isoforms.
Cellular localizationGolgi Apparatus.
- FLJ12492 antibody
- FLJ14629 antibody
- FLJ14801 antibody
All lanes : Anti-OSBPL9 antibody [EPR9333] (ab151691) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 83 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling OSBPL9 with ab151691 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow cytometric analysis of permeabilized 293T cells labeling OSBPL9 with ab151691 at 1/10 dilution (red) compared to a nonspecific control antibody (green).
ab151691 has been referenced in 1 publication.
- Gillingham AK et al. In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation. Elife 8:N/A (2019). PubMed: 31294692