Overview

  • Product name

    Anti-OSBPL9 antibody [EPR9333]
    See all OSBPL9 primary antibodies
  • Description

    Rabbit monoclonal [EPR9333] to OSBPL9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human OSBPL9 aa 650-750. The exact sequence is proprietary.

  • Positive control

    • Human fetal brain, Jurkat, 293T and HepG2 cell lysates; Human kidney tissue; 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    The OSBPL9 gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. OSBPL9 appears to be in a distinct OSBP subfamily and shares relatively little homology in the sterol-binding domain with other OSBPs. Multiple transcript variants have been identified, most of which encode distinct isoforms.
  • Cellular localization

    Golgi Apparatus.
  • Database links

  • Alternative names

    • FLJ12492 antibody
    • FLJ14629 antibody
    • FLJ14801 antibody
    • FLJ32055 antibody
    • FLJ34384 antibody
    • MGC15035 antibody
    • ORP9 antibody
    • OSBP related protein 9 antibody
    • OSBP4 antibody
    • Oxysterol binding protein like 9 antibody
    • Oxysterol binding protein related protein 9 antibody
    • oxysterol binding protein-like 9 antibody
    • oxysterol binding proteinlike9 antibody
    see all

Images

  • All lanes : Anti-OSBPL9 antibody [EPR9333] (ab151691) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 83 kDa

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling OSBPL9 with ab151691 at 1/50 dilution.
  • Flow cytometric analysis of permeabilized 293T cells labeling OSBPL9 with ab151691 at 1/10 dilution (red) compared to a nonspecific control antibody (green).

References

ab151691 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HEK cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jun 15 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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