Recombinant Anti-OSBPL9 antibody [EPR9333] (ab151691)
![Western blot - Anti-OSBPL9 antibody [EPR9333] (ab151691)](https://www.abcam.com/ps/products/151/ab151691/Images/ab151691-158703-anti-osbpl9-antibody-epr9333-western-blot.jpg "Western blot - Anti-OSBPL9 antibody [EPR9333] (ab151691)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9333] to OSBPL9
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-OSBPL9 antibody [EPR9333]
See all OSBPL9 primary antibodies -
Description
Rabbit monoclonal [EPR9333] to OSBPL9 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human OSBPL9 aa 650-750. The exact sequence is proprietary.
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Positive control
- Human fetal brain, Jurkat, 293T and HepG2 cell lysates; Human kidney tissue; 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9333 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 83 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
The OSBPL9 gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. OSBPL9 appears to be in a distinct OSBP subfamily and shares relatively little homology in the sterol-binding domain with other OSBPs. Multiple transcript variants have been identified, most of which encode distinct isoforms. -
Cellular localization
Golgi Apparatus. -
Database links
- Entrez Gene: 114883 Human
- Entrez Gene: 100273 Mouse
- Entrez Gene: 298369 Rat
- Omim: 606737 Human
- SwissProt: Q96SU4 Human
- SwissProt: A2A8Z1 Mouse
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Alternative names
- FLJ12492 antibody
- FLJ14629 antibody
- FLJ14801 antibody
see all
Images
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Western blot - Anti-OSBPL9 antibody [EPR9333] (ab151691)All lanes : Anti-OSBPL9 antibody [EPR9333] (ab151691) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 83 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL9 antibody [EPR9333] (ab151691)](https://www.abcam.com/ps/products/151/ab151691/Images/ab151691-158702-anti-osbpl9-antibody-epr9333-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL9 antibody [EPR9333] (ab151691)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling OSBPL9 with ab151691 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-OSBPL9 antibody [EPR9333] (ab151691)](https://www.abcam.com/ps/products/151/ab151691/Images/ab151691-158701-anti-osbpl9-antibody-epr9333-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-OSBPL9 antibody [EPR9333] (ab151691)Intracellular flow cytometric analysis of permeabilized 293T cells labeling OSBPL9 with ab151691 at 1/10 dilution (red) compared to a nonspecific control antibody (green).
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![Anti-OSBPL9 antibody [EPR9333] (ab151691)](https://www.abcam.com/ps/products/151/ab151691/Images/ab151691-4-benefits-of-recombinant-antibodies.png)
Anti-OSBPL9 antibody [EPR9333] (ab151691)
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab151691 has been referenced in 1 publication.
- Gillingham AK et al. In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation. Elife 8:N/A (2019). PubMed: 31294692
