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  1. Link

    osbpl9-antibody-epr9333-ab151691.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
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RecombinantRabMAb

Recombinant Anti-OSBPL9 antibody [EPR9333] (ab151691)

  • Datasheet
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Western blot - Anti-OSBPL9 antibody [EPR9333] (ab151691)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL9 antibody [EPR9333] (ab151691)
  • Flow Cytometry (Intracellular) - Anti-OSBPL9 antibody [EPR9333] (ab151691)
  • Anti-OSBPL9 antibody [EPR9333] (ab151691)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9333] to OSBPL9
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-OSBPL9 antibody [EPR9333]
    See all OSBPL9 primary antibodies
  • Description

    Rabbit monoclonal [EPR9333] to OSBPL9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human OSBPL9 aa 650-750. The exact sequence is proprietary.

  • Positive control

    • Human fetal brain, Jurkat, 293T and HepG2 cell lysates; Human kidney tissue; 293T cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9333
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism

Associated products

  • Alternative Versions

    • Anti-OSBPL9 antibody [EPR9333] - BSA and Azide free (ab249015)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human OSBPL9 protein (ab165052)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (1)
1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance

    The OSBPL9 gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. OSBPL9 appears to be in a distinct OSBP subfamily and shares relatively little homology in the sterol-binding domain with other OSBPs. Multiple transcript variants have been identified, most of which encode distinct isoforms.
  • Cellular localization

    Golgi Apparatus.
  • Database links

    • Entrez Gene: 114883 Human
    • Entrez Gene: 100273 Mouse
    • Entrez Gene: 298369 Rat
    • Omim: 606737 Human
    • SwissProt: Q96SU4 Human
    • SwissProt: A2A8Z1 Mouse
    • Alternative names

      • FLJ12492 antibody
      • FLJ14629 antibody
      • FLJ14801 antibody
      • FLJ32055 antibody
      • FLJ34384 antibody
      • MGC15035 antibody
      • ORP9 antibody
      • OSBP related protein 9 antibody
      • OSBP4 antibody
      • Oxysterol binding protein like 9 antibody
      • Oxysterol binding protein related protein 9 antibody
      • oxysterol binding protein-like 9 antibody
      • oxysterol binding proteinlike9 antibody
      see all

    Images

    • Western blot - Anti-OSBPL9 antibody [EPR9333] (ab151691)
      Western blot - Anti-OSBPL9 antibody [EPR9333] (ab151691)
      All lanes : Anti-OSBPL9 antibody [EPR9333] (ab151691) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 83 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL9 antibody [EPR9333] (ab151691)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL9 antibody [EPR9333] (ab151691)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling OSBPL9 with ab151691 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-OSBPL9 antibody [EPR9333] (ab151691)
      Flow Cytometry (Intracellular) - Anti-OSBPL9 antibody [EPR9333] (ab151691)

      Intracellular flow cytometric analysis of permeabilized 293T cells labeling OSBPL9 with ab151691 at 1/10 dilution (red) compared to a nonspecific control antibody (green).

       

    • Anti-OSBPL9 antibody [EPR9333] (ab151691)
      Anti-OSBPL9 antibody [EPR9333] (ab151691)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab151691? Please let us know so that we can cite the reference in this datasheet.

    ab151691 has been referenced in 1 publication.

    • Gillingham AK  et al. In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation. Elife 8:N/A (2019). PubMed: 31294692

    Customer reviews and Q&As

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    Western blot abreview for Anti-OSBPL9 antibody [EPR9333]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    HEK cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 15 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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