Recombinant Anti-OSMR antibody [EPR24786-50] (ab273103)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24786-50] to OSMR
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-OSMR antibody [EPR24786-50]
See all OSMR primary antibodies -
Description
Rabbit monoclonal [EPR24786-50] to OSMR -
Host species
Rabbit -
Specificity
- WB suitable for mouse only
- FC and IP not suitable for human and mouse
- ICC not suitable for human
- IHC not suitable for human, mouse and rat
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 3T3-L1 and NIH/3T3 whole cell lysate. Mouse lung tissue lysate. PNGase F treated/untreated 3T3-L1 and NIH/3T3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24786-50 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 111 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 111 kDa. |
Target
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Function
Associates with IL31RA to form the IL31 receptor. Binds IL31 to activate STAT3 and possibly STAT1 and STAT5. Capable of transducing OSM-specific signaling events. -
Tissue specificity
Expressed at relatively high levels in all neural cells as well as fibroblast, epithelial and a variety of tumor cell lines. -
Involvement in disease
Amyloidosis, primary localized cutaneous, 1 (PLCA1) [MIM:105250]: A primary amyloidosis characterized by localized cutaneous amyloid deposition. This condition usually presents with itching (especially on the lower legs) and visible changes of skin hyperpigmentation and thickening that may be exacerbated by chronic scratching and rubbing. Primary localized cutaneous amyloidosis is often divided into macular and lichen subtypes although many affected individuals often show both variants coexisting. Lichen amyloidosis characteristically presents as a pruritic eruption of grouped hyperkeratotic papules with a predilection for the shins, calves, ankles and dorsa of feet and thighs. Papules may coalesce to form hyperkeratotic plaques that can resemble lichen planus, lichen simplex or nodular prurigo. Macular amyloidosis is characterized by small pigmented macules that may merge to produce macular hyperpigmentation, sometimes with a reticulate or rippled pattern. In macular and lichen amyloidosis, amyloid is deposited in the papillary dermis in association with grouped colloid bodies, thought to represent degenerate basal keratinocytes. The amyloid deposits probably reflect a combination of degenerate keratin filaments, serum amyloid P component, and deposition of immunoglobulins. Note=The disease is caused by mutations affecting the gene represented in this entry. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 4 fibronectin type-III domains. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 18414 Mouse
- SwissProt: O70458 Mouse
- Unigene: 10760 Mouse
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Alternative names
- IL-31 receptor subunit beta antibody
- IL-31R subunit beta antibody
- IL-31R-beta antibody
see all
Images
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All lanes : Anti-OSMR antibody [EPR24786-50] (ab273103) at 1/1000 dilution
Lane 1 : 3T3-L1 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3 : M1 (mouse myeloid leukemia myeloblast) whole cell lysate
Lane 4 : EL4 (mouse lymphoma T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 111 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The observed MW are consistent with what has been described in the literature (PMID: 27325693).
Negative control: M1, EL4 (PMID: 9920829).
Exposure time: 15 seconds.
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Anti-OSMR antibody [EPR24786-50] (ab273103) at 1/1000 dilution + Mouse lung tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 111 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The observed MW are consistent with what has been described in the literature (PMID: 26311783).
Exposure time: 3 minutes.
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All lanes : Anti-OSMR antibody [EPR24786-50] (ab273103) at 1/1000 dilution
Lane 1 : Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : 3T3-L1 whole cell lysate treated with PNGase F
Lane 3 : Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : NIH/3T3 whole cell lysate treated with PNGase F
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 111 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
OSMR is a glycosylated protein and can be deglycosylated by PNGase F.
The observed MW are consistent with what has been described in the literature (PMID: 26311783).
Exposure time: 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab273103 has not yet been referenced specifically in any publications.