Overview

  • Product name

  • Description

    Rabbit polyclonal to Osteocalcin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Osteocalcin. (Near the C terminal; NP_954642.1).
    Database link: P02818

  • Positive control

    • Human breast cancer and ovarian cancer tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab198228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.

Target

  • Function

    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • Sequence similarities

    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    modifications

    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Osteocalcin with ab198228 at 1/60 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Osteocalcin with ab198228 at 1/60 dilution.

References

This product has been referenced in:

  • Zhang ZZ  et al. 3D printed poly(e-caprolactone) scaffolds function with simvastatin-loaded poly(lactic-co-glycolic acid) microspheres to repair load-bearing segmental bone defects. Exp Ther Med 17:79-90 (2019). Read more (PubMed: 30651767) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Vetor Unmasking solution
Permeabilization
Yes - 0.05% triton-x100
Specification
Bone
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 28 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (femur)
Permeabilization
No
Specification
femur
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde

Dr. Hanson Lee

Verified customer

Submitted Aug 27 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (tooth)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Vector antigen unmask solution
Permeabilization
Yes - triton
Specification
tooth
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

Submitted Jun 16 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (growth plate)
Permeabilization
No
Specification
growth plate
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

Submitted Jun 16 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (alveolar bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution, Tris-Based
Permeabilization
Yes - Triton X
Specification
alveolar bone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

Submitted Jan 16 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Molar)
Permeabilization
Yes - Triton X-100
Specification
Molar
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

Submitted Apr 25 2017

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