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  1. Link

    osteocalcin-antibody-ab198228.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Bone
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Anti-Osteocalcin antibody (ab198228)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (4)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteocalcin antibody (ab198228)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteocalcin antibody (ab198228)

Key features and details

  • Rabbit polyclonal to Osteocalcin
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Protein
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Recombinant Human Osteocalcin protein (ab152231)
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Overview

  • Product name

    Anti-Osteocalcin antibody
    See all Osteocalcin primary antibodies
  • Description

    Rabbit polyclonal to Osteocalcin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Osteocalcin. (Near the C terminal; NP_954642.1).
    Database link: P02818

  • Positive control

    • Human breast cancer and ovarian cancer tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Osteocalcin protein (ab152231)
  • Related Products

    • Recombinant Human Osteocalcin protein (ab152231)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab198228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
1/50 - 1/200.
Notes
IHC-P
1/50 - 1/200.

Target

  • Function

    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • Sequence similarities

    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    modifications

    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02818 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 632 Human
    • Omim: 112260 Human
    • SwissProt: P02818 Human
    • Unigene: 654541 Human
    • Alternative names

      • BGLAP antibody
      • BGP antibody
      • Bone gamma carboxyglutamate (gla) protein antibody
      • Bone gamma carboxyglutamate gla protein osteocalcin antibody
      • Bone gamma carboxyglutamate protein antibody
      • Bone Gla protein antibody
      • Gamma carboxyglutamic acid containing protein antibody
      • Gamma-carboxyglutamic acid-containing protein antibody
      • OC antibody
      • OCN antibody
      • OSTCN_HUMAN antibody
      • Osteocalcin antibody
      • OTTHUMP00000016586 antibody
      • PMF1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteocalcin antibody (ab198228)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteocalcin antibody (ab198228)

      Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Osteocalcin with ab198228 at 1/60 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteocalcin antibody (ab198228)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteocalcin antibody (ab198228)

      Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Osteocalcin with ab198228 at 1/60 dilution.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab198228? Please let us know so that we can cite the reference in this datasheet.

    ab198228 has been referenced in 4 publications.

    • Wu CY  et al. Vapor-phased fabrication and modulation of cell-laden scaffolding materials. Nat Commun 12:3413 (2021). PubMed: 34099701
    • Shen J  et al. EGFL6 regulates angiogenesis and osteogenesis in distraction osteogenesis via Wnt/ß-catenin signaling. Stem Cell Res Ther 12:415 (2021). PubMed: 34294121
    • Zhang ZZ  et al. 3D printed poly(e-caprolactone) scaffolds function with simvastatin-loaded poly(lactic-co-glycolic acid) microspheres to repair load-bearing segmental bone defects. Exp Ther Med 17:79-90 (2019). PubMed: 30651767
    • Wang L  et al. Periosteal Mesenchymal Progenitor Dysfunction and Extraskeletally-Derived Fibrosis Contribute to Atrophic Fracture Nonunion. J Bone Miner Res 34:520-532 (2019). PubMed: 30602062

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Osteocalcin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (femur)
    Permeabilization
    No
    Specification
    femur
    Blocking step
    Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Hanson Lee

    Verified customer

    Submitted Aug 27 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Osteocalcin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (tooth)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Vector antigen unmask solution
    Permeabilization
    Yes - triton
    Specification
    tooth
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Tina Yuan

    Verified customer

    Submitted Jun 16 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Osteocalcin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (growth plate)
    Permeabilization
    No
    Specification
    growth plate
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Tina Yuan

    Verified customer

    Submitted Jun 16 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Osteocalcin antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (alveolar bone)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution, Tris-Based
    Permeabilization
    Yes - Triton X
    Specification
    alveolar bone
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Tina Yuan

    Verified customer

    Submitted Jan 16 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Osteocalcin antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Molar)
    Permeabilization
    Yes - Triton X-100
    Specification
    Molar
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Tina Yuan

    Verified customer

    Submitted Apr 25 2017

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