Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (femur)
Permeabilization
No
Specification
femur
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking solution
Dilution
1/100
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Thermo Fisher Goat anti-Rabbit IgG (H+L) Cross-Ads
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Hanson Lee
Verified customer
Submitted Aug 27 2019