Overview

  • Product name

    Anti-Osteocalcin antibody [EPR3690]
    See all Osteocalcin primary antibodies
  • Description

    Rabbit monoclonal [EPR3690] to Osteocalcin
  • Host species

    Rabbit
  • Specificity

    This antibody fails to detect endogenous Osteocalcin in cell lines and tissues tested. So we don`t guarantee WB in natural samples.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Osteocalcin aa 50-150. The exact sequence is proprietary.
    Database link: P02818

  • Positive control

    • WB: Human Osteocalcin recombinant protein
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.

This antibody fails to detect endogenous Osteocalcin in cell lines and tissues tested. So we don`t guarantee WB in natural samples.

Target

  • Function

    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • Sequence similarities

    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    modifications

    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all

Images

  • Anti-Osteocalcin antibody [EPR3690] (ab133612) at 1.2 µg/ml (purified) + Human osteocalcin recombinant protein at 0.015 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 11 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Anti-Osteocalcin antibody [EPR3690] (ab133612) at 1/1000 dilution (Unpurified) + Human Osteocalcin recombinant protein at 0.01 µg

    Predicted band size: 11 kDa

References

This product has been referenced in:

  • Bian Y  et al. Human amnion-derived mesenchymal stem cells promote osteogenesis of human bone marrow mesenchymal stem cells against glucolipotoxicity. FEBS Open Bio 9:74-81 (2019). Read more (PubMed: 30652075) »
  • Mirzaei A  et al. Comparison of osteogenic differentiation potential of induced pluripotent stem cells on 2D and 3D polyvinylidene fluoride scaffolds. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30851069) »
See all 11 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab133612.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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