Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3690] to Osteocalcin
- Suitable for: WB
- Reacts with: Human
Product nameAnti-Osteocalcin antibody [EPR3690]
See all Osteocalcin primary antibodies
DescriptionRabbit monoclonal [EPR3690] to Osteocalcin
SpecificityThis antibody fails to detect endogenous Osteocalcin in cell lines and tissues tested. So we don`t guarantee WB in natural samples.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Osteocalcin aa 50-150. The exact sequence is proprietary.
Database link: P02818
- WB: Human Osteocalcin recombinant protein
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab133612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
This antibody fails to detect endogenous Osteocalcin in cell lines and tissues tested. So we don`t guarantee WB in natural samples.
FunctionConstitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Sequence similaritiesBelongs to the osteocalcin/matrix Gla protein family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
- Information by UniProt
- BGLAP antibody
- BGP antibody
- Bone gamma carboxyglutamate (gla) protein antibody
Anti-Osteocalcin antibody [EPR3690] (ab133612) at 1.2 µg/ml (purified) + Human osteocalcin recombinant protein at 0.015 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
Anti-Osteocalcin antibody [EPR3690] (ab133612) at 1/1000 dilution (Unpurified) + Human Osteocalcin recombinant protein at 0.01 µg
Predicted band size: 11 kDa
ab133612 has been referenced in 12 publications.
- Liu Z et al. Multiple myeloma-derived exosomes inhibit osteoblastic differentiation and improve IL-6 secretion of BMSCs from multiple myeloma. J Investig Med 68:45-51 (2020). PubMed: 31784427
- Bian Y et al. Human amnion-derived mesenchymal stem cells promote osteogenesis of human bone marrow mesenchymal stem cells against glucolipotoxicity. FEBS Open Bio 9:74-81 (2019). PubMed: 30652075
- Mirzaei A et al. Comparison of osteogenic differentiation potential of induced pluripotent stem cells on 2D and 3D polyvinylidene fluoride scaffolds. J Cell Physiol N/A:N/A (2019). PubMed: 30851069
- Shi R et al. Analysis of the characteristics and expression profiles of coding and noncoding RNAs of human dental pulp stem cells in hypoxic conditions. Stem Cell Res Ther 10:89 (2019). PubMed: 30867055
- Pei DD et al. Contribution of Mitophagy to Cell-Mediated Mineralization: Revisiting a 50-Year-Old Conundrum. Adv Sci (Weinh) 5:1800873 (2018). PubMed: 30356983