Overview

  • Product name
    Anti-Osteocalcin antibody [OC4-30]
    See all Osteocalcin primary antibodies
  • Description
    Mouse monoclonal [OC4-30] to Osteocalcin
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-Fr, Sandwich ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Sheep, Rabbit, Goat, Cow, Dog, Human, Pig
    Does not react with: Mouse
  • Immunogen

    Full length protein corresponding to Cow Osteocalcin.
    Database link: P02820

  • Epitope
    Includes the gamma-carboxylated residue at position 17.
  • Positive control
    • IHC-Fr: Human bone marrow tissue. ICC/IF: Human dental pulp stem cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab13418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 12.0 kDa.
IHC-Fr Use a concentration of 10 µg/ml.
Sandwich ELISA Use a concentration of 1 - 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20537381
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • Sequence similarities
    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    modifications
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all

Images

  • ab13418 staining Osteocalcin in Human bone marrow stem cells tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was blocked with 5%BSA + TBS  + 0,5%Tween for 10 minutes. Samples were incubated with primary antibody (1/80) for 14 hours. An undiluted Biotin-conjugated Donkey polyclonal was used as the secondary antibody.

    See Abreview

  • Immunofluorescence analysis of 1 month osteoblastic-differentiated Human dental pulp stem cells, staining Osteocalcin with ab13418 at 1/80 dilution. A FITC-conjugated anti-mouse IgG was used as the secondary antibody.

References

This product has been referenced in:
  • He W  et al. Effects of silica-gentamicin nanohybrids on osteogenic differentiation of human osteoblast-like SaOS-2 cells. Int J Nanomedicine 13:877-893 (2018). Read more (PubMed: 29445277) »
  • Liu H  et al. Endothelial progenitor cells improve the therapeutic effect of mesenchymal stem cell sheets on irradiated bone defect repair in a rat model. J Transl Med 16:137 (2018). Read more (PubMed: 29788957) »
See all 47 Publications for this product

Customer reviews and Q&As

1-10 of 34 Abreviews or Q&A

Answer



Unfortunately, none of the antibodies requested have been tested in sandwich ELISA. They are validated for either direct or indirect ELISA, but they have not been paired to another antibody to work together in the application.

However, I can suggest alternative products which are guaranteed for sandwich ELISA. For example, against IGF1, the antibody https://www.abcam.com/igf1-antibody-ab9572.html can be used as capture antibody paired with https://www.abcam.com/ab83137.html biotynilated.

Similarly, antibody ab130754 is validated in this application but I may need to check with the lab which detection antibody was used with it.

I could not find in our catalogue a pair of antibodies against Osteocalcin, as all the alternatives available are suitable for direct / indirect ELISA.

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Answer

Gracias por contactarnos.

Tenemos en el catálogo, como bien indicas, tres anticuerpos contra Osteocalcina testados en humano mediante inmunocitoquímica e immunohistoquímica: ab13418, ab13420 y ab13421.

Todos nuestros productos tienen 6 meses de garantía, y en caso de no dar la tinción esperada en las aplicaciones y especies indicadas en la datasheet se os reembolsaría el importe del mismo, o se os reemplazaría el vial.

En cuanto a la forma que reconocen, estos anticuerpos reconocen tanto la forma intracelular como la secretada.

Para hacer el pedido, si lo haces desde España puedes mandarnos un mail a mailto:orders@abcam.com o llamarnos al 91 114 65 54 indicando dirección de envío y facturación, teléfono de contacto y un CIF validado para operaciones intracomunitarias. El pago puede hacerse sin ningún problema por transferencia bancaria.

Espero haber resuelto tus dudas, si tuvieras cualquier otra consulta, no dudes en contactarme de nuevo.

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Answer

Thank you for contacting us.

Please buy ab7097.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

Once the incubation with secondary antibody is finished then you need to add tertiary antibody which is in your case would be anti rat IgG-HRP. Please incubate for 1 hour at room temperature. Please make sure the tertiary antibody is HRP conjugated.

The only positive thing about using tertiary antibody is the signal amplification. Else you can do the experiment without it by using correct conjugated secondary antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for contacting us.

We have a Trypsin Enzymatic Antigen Retrieval Solution in our catalogue with reference ab970 (https://www.abcam.com/ab970).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

The protocol seems fine to me. However the following updates can be tried

- 0.3% H2O2 can be incubated for 10-15 minutes only.
- The endogenous peroxidases can be blocked after addition of primary antibody. Please check whether the tissue sections have active endogenous perxidases in it. Please follow the guidelines in section v of protocol (https://www.abcam.com/index.html?pageconfig=resource&rid=11494#v)

- ab79091 rat secondary antibody is a un-conjugated product. This will not be giving you any staining with DAB.

Are you using any anti rat HRP polymer for detection?

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Answer

Thank you for contacting us.

I have asked few questions in my previous email so please send be answers asap. It would be very helpful if you could send me your step by step protocol as I suspect the protocol might needs updates.

Looking forward to hearing from you soon.

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Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

- The antigen retrieval method always needs optimizations. Could you tell us for how long the tissues sections were treated in microwave or pressure cooker?
- What was the pH of citrate buffer? For how long the tissue were fixed?
- What is the purchase order number and how the antibody was stored?
- Does the detection system work fine? Have you tired this with other antibodies?

Please note the ab79091 is an un-conjugated antibody so before using it in IHC it should either be conjugated or a tertiary anti rat antibody should be used. Could you clarify if you have used the tertiary antibody else you should not expect any staining?

To get good IHC results bone sections normally needs to be decalcified. Have you tried the decalcification step?

Thank you very much for your cooperation. I will look forward to hearing from you soon.

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Answer

This is correct.
Please note that some antibodies work in both IHC-P and IHC-GMA, some do work in IHC-P or IHC-GMA only.
I hope this helps.

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Answer

Yes we have a recommended protocol for resin (GMA) embedding.
The link to the protocol is: https://www.abcam.com/index.html?pageconfig=resource&rid=11464.
Note that you don't need the antigen retrieval step in your IHC protocol when working with acetone fixed and GMA embedded tissues. GMA stands for Glycol Methacrylate.

I hope this is helpful.

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1-10 of 34 Abreviews or Q&A

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