Product nameAnti-Osteocalcin antibody [OCG3]
See all Osteocalcin primary antibodies
DescriptionMouse monoclonal [OCG3] to Osteocalcin
Tested applicationsSuitable for: WB, IHC-Fr, IHC-P, Sandwich ELISA, ICC/IFmore details
Species reactivityReacts with: Rat, Sheep, Rabbit, Goat, Chicken, Cow, Dog, Human, Pig
Does not react with: Mouse
Full length protein corresponding to Cow Osteocalcin.
Database link: P02820
- Normal bovine bone tissue
Though we have found ab13420 does not react with mouse sample in our lab, there is a report from a reviewer that got positive result with mouse sample in IHC-Fr. However, this information needs to be confirmed further.
Although this antibody does work in IHC-P, we have found that ab13418 typically gives a more robust signal. Therefore, if you plan on using this antibody in IHC-P, we recommend ab13418 as an alternative option.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab13420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 12 kDa.
Use under reducing or non-reducing conditions.
|IHC-Fr||Use a concentration of 1 - 10 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml. Enzymatic retrieval with Proteinase K is recommended.|
|Sandwich ELISA||Use a concentration of 1 - 10 µg/ml.
For use in sandwich ELISA with ab13421
|ICC/IF||Use at an assay dependent concentration. PubMed: 21396708|
FunctionConstitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Sequence similaritiesBelongs to the osteocalcin/matrix Gla protein family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
- Information by UniProt
- BGLAP antibody
- BGP antibody
- Bone gamma carboxyglutamate (gla) protein antibody
This product has been referenced in:
- Zhou D et al. Valproic acid prevents glucocorticoid-induced osteonecrosis of the femoral head of rats. Int J Mol Med 41:3433-3447 (2018). Read more (PubMed: 29512684) »
- Zhang J et al. Repair of Critical-Sized Mandible Defects in Aged Rat Using Hypoxia Preconditioned BMSCs with Up-regulation of Hif-1a. Int J Biol Sci 14:449-460 (2018). Read more (PubMed: 29725266) »