Overview

  • Product name
    Anti-Osteocalcin antibody [OCG3]
    See all Osteocalcin primary antibodies
  • Description
    Mouse monoclonal [OCG3] to Osteocalcin
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-P, Sandwich ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Sheep, Rabbit, Goat, Chicken, Cow, Dog, Human, Pig
    Does not react with: Mouse
  • Immunogen

    Full length protein corresponding to Cow Osteocalcin.
    Database link: P02820

  • Epitope
    Residues 21-31
  • Positive control
    • Normal bovine bone tissue
  • General notes

    Though we have found ab13420 does not react with mouse sample in our lab, there is a report from a reviewer that got positive result with mouse sample in IHC-Fr. However, this information needs to be confirmed further.

     

    Although this antibody does work in IHC-P, we have found that ab13418 typically gives a more robust signal. Therefore, if you plan on using this antibody in IHC-P, we recommend ab13418 as an alternative option.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab13420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 12 kDa.

Use under reducing or non-reducing conditions.

IHC-Fr Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Enzymatic retrieval with Proteinase K is recommended.
Sandwich ELISA Use a concentration of 1 - 10 µg/ml.

For use in sandwich ELISA with ab13421

ICC/IF Use at an assay dependent concentration. PubMed: 21396708

Target

  • Function
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • Sequence similarities
    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    modifications
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all

Images

  • Detection of human osteocalcin by ab13420
  • Image from Strauß S et al., PloS One. 2012;7(12):14. Fig 8.; doi: 10.1371/journal.pone.0051264

References

This product has been referenced in:
  • Zhou D  et al. Valproic acid prevents glucocorticoid-induced osteonecrosis of the femoral head of rats. Int J Mol Med 41:3433-3447 (2018). Read more (PubMed: 29512684) »
  • Zhang J  et al. Repair of Critical-Sized Mandible Defects in Aged Rat Using Hypoxia Preconditioned BMSCs with Up-regulation of Hif-1a. Int J Biol Sci 14:449-460 (2018). Read more (PubMed: 29725266) »
See all 46 Publications for this product

Customer reviews and Q&As

1-10 of 26 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Human Cultured Cells (PULP)
Permeabilization
No
Specification
PULP
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde

Kyungjoo Seong

Verified customer

Submitted Nov 30 2016

Answer

Gracias por contactarnos.

Tenemos en el catálogo, como bien indicas, tres anticuerpos contra Osteocalcina testados en humano mediante inmunocitoquímica e immunohistoquímica: ab13418, ab13420 y ab13421.

Todos nuestros productos tienen 6 meses de garantía, y en caso de no dar la tinción esperada en las aplicaciones y especies indicadas en la datasheet se os reembolsaría el importe del mismo, o se os reemplazaría el vial.

En cuanto a la forma que reconocen, estos anticuerpos reconocen tanto la forma intracelular como la secretada.

Para hacer el pedido, si lo haces desde España puedes mandarnos un mail a mailto:orders@abcam.com o llamarnos al 91 114 65 54 indicando dirección de envío y facturación, teléfono de contacto y un CIF validado para operaciones intracomunitarias. El pago puede hacerse sin ningún problema por transferencia bancaria.

Espero haber resuelto tus dudas, si tuvieras cualquier otra consulta, no dudes en contactarme de nuevo.

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Answer

Thank you very much for contacting us with your enquiry.

The AMELX protein ab139212 can be diluted in any buffer. However, all of our products are intended for research use only and are not recommended for in vitro studies. This protein contains DTT, glycerol, and Tris HCL in its buffer, and it is not a sterile product, so we do not suggest it for use in a live animal study.

Ab13420 and ab90395 are mouse monoclonal antibodies, so they can be detected with a detection kit like ab94743-

https://www.abcam.com/EXPOSE-Mouse-Specific-AP-red-Detection-IHC-Kit-ab94743.html

I hope that this information will be useful, but please let me know if you have further questions or need anything else, and I'll be happy to help.

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Answer

Vielen Dank für Ihre Anfrage.

Die Lieferung über das Wochenende erfolgt bei uns regelmaessig, ist also kein Grund zur Beunruhigung, da wir erstens den Antikörper mit Kuhlakkus versenden, zweitens FedEx die Produkte im Kühlhaus lagert und drittens Antikörper sehr stabile Proteine sind. So versenden wir unsere Produkte ja auch nach Ost-Asien und Australien, und da sind die schon mal locker eine Woche unterwegs.

Wir haben interne Versuche zur Aktivität unter verschieden Lagerbedingungen durchgeführt, und wir festgestellt, dass die meisten Antikörper sogar bei einer Lagerung für drei Monate bei Raumtemperatur ihre Aktivität nicht verlieren!

Wir testen äusserst selten Antikörper in anderen Applikationen als IHC-P oder IHC-F für die Immunohistochemie. Daher kann ich Ihnen leider nicht garantieren, ob unsere Antikörper mit dem technovit 9100neu Einbettugssystem funktionieren.

Hinsichtlich der zwei Antikörper kann ich Ihnen auf den ersten Blick sagen, dass der eine Antikörper aufgereinigt wurde (ab75043), und der andere nicht (ab75078). Desweiteren hat der ab75043 eine recht niedrige Affinität, da er in sehr hohen Konzentrationen eingesetzt werden muss.

Eine Positivkontrolle versenden wir leider nicht mit unseren Produkten. Und ja, die meistens unserer In-Haus Antikörper werden tatsächlich mit jedem neuen Lot aufs neue getestet, so dass unsere Datenblätter immer aktuell sind.



Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issuedfree of charge replacements with the following order numbers:


replacement order number xxx with ab81357
replacement order number xxx with ab13418
replacement order number xxx with ab133580. This item is backordered, and the estimated delivery date is xxx.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Answer

Thank you for contacting us.

I am sorry to hear about the shortage and sincerely apologize about that.


Your free of charge replacement for ab13420 will be shipped with your next order. The free replacement is shipping at full price, but credit note xxx has been raised to offset the charge.

The credit notecan be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Question
Answer

Thank you very much for your email.

I had sent a reply to this on Wednesday, but it seems that it did not get to your inbox. Could you please let me know once you receive this message? My previous message is below:

"Thank you very much for contacting us with your question.

The osteocalcin antibody ab13420 is provided at a concentration of 2 mg/mL, and we recommend using the antibody at 10 ug/mL in IHC-P. So, the antibody will need to be diluted 1:200 before use.

For example, if you need to use a total of 400 uL of antibody solution, you would add 2 uL of the provided antibody solution into 398 uL of diluent such as TBST.

I hope that this information will be useful, but if you have further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help."

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Answer

Thank you very much for contacting us with your question.

The osteocalcin antibody ab13420 is provided at a concentration of 2 mg/mL, and we recommend using the antibody at 10 ug/mL in IHC-P. So, the antibody will need to be diluted 1:200 before use.

For example, if you need to use a total of 400 uL of antibody solution, you would add 2 uL of the provided antibody solution into 398 uL of diluent such as TBST.

I hope that this information will be useful, but if you have further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

Have a nice day.

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Answer

DISCOUNT CODE: XXXXXXXXXX
Expiration date: 21-09-2012

I am very pleased to hear you would like to accept our offer and test ab13420 in [GUNIEA PIG]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for [Guniea Pig] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer


Nous somme vraiment désolés de ne pas avoir pu vous fournir un bon anticorps anti-osteocalcine. J'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.

Votre référence CN est ******.

Voici les manières dont vous pouvez utiliser cette référence CN:
(1) la faire valoir contre la facture originale si celle-ci n'as pas encore été payée
(2) la faire valoir contre une prochaine facture
(3) obtenir un remboursement de la somme entière si vous n'avez pas de factures actuellement en cours avec Abcam

Si vous souhaitez recevoir un remboursement au lieu d'un avoir, veuillez demander à votre service de gestion de prendre contact avec notre département comptabilité afin que nous puissions recueillir les informations nécessaires pour cette restitution. Notre service comptabilité peut être contacté par courriel à l'adresse creditcontrol@abcam.com ou par téléphone en utilisant le lien « Contactez-nous » dans le coin en haut à droite de notre site internet. Veuillez communiquer votre numéro d'avoir lorsque vous contactez notre service comptabilité.

Vous recevrez également la référence complète de la note de crédit par courriel ou par voie postale; cette référence commençant par les lettres CGB.

J'espère que cette expérience ne vous empêchera pas de commander à nouveau l'un de nos produits.

L'équipe technique reste à votre disposition pour plus de conseils.


Bonne continuation dans votre recherche.

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1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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