• Product name
    Anti-Osteocalcin antibody [OCG4]
    See all Osteocalcin primary antibodies
  • Description
    Mouse monoclonal [OCG4] to Osteocalcin
  • Host species
  • Tested applications
    Suitable for: ELISA, WB, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Sheep, Rabbit, Goat, Chicken, Cow, Dog, Human, Pig
    Does not react with: Mouse, Rat
  • Immunogen

    Full length protein corresponding to Cow Osteocalcin.
    Database link: P02820

  • Epitope
    Residues 21-31
  • Positive control
    • ICC/IF: SKNSH cells.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 



Our Abpromise guarantee covers the use of ab13421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1 - 1/10.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 11 kDa.
IHC-Fr Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 1 - 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 16820635


  • Function
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • Sequence similarities
    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all


  • ICC/IF image of ab13421 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12421, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Immunohistochemistry of human Synovial Sarcoma staining Osteocalcin with ab13421

  • Immunohistochemistry of human Synovial Sarcoma staining Osteocalcin with ab13421

  • Immunohistochemistry of human Chondroma staining Osteocalcin with ab13421


This product has been referenced in:
  • Zou L  et al. Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells. Sci Rep 6:22868 (2016). IHC . Read more (PubMed: 26980556) »
  • González S  et al. A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells. Stem Cell Res 16:358-64 (2016). Read more (PubMed: 26896856) »

See all 6 Publications for this product

Customer reviews and Q&As

Gracias por contactarnos.

Tenemos en el catálogo, como bien indicas, tres anticuerpos contra Osteocalcina testados en humano mediante inmunocitoquímica e immunohistoquímica: ab13418, ab13420 y ab13421.

Todos nue...

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Nous somme vraiment désolés de ne pas avoir pu vous fournir un bon anticorps anti-osteocalcine. J'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.

Votre référence CN est *******.

Voici les man...

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Merci d'avoir répondu à notre questionnaire.

Je suis désolé d'apprendre que vous rencontrez toujours des problèmes avec l'anticorps anti-osteocalcin ab13421 que je vous ai envoyé en remplacement du ab...

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Thank you for your patience.

I have received the following information about the other 3 osteocalcin antibodies I refered to in my last email:

ab13418, ab13421: These antibodies were derived from mice that were immunized with ...

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Thank you for your phone call.

I have checked our osteocalcin antibodies and found one where I know for sure that it was raised against the mature human protein without any modification: ab35078

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That is correct. However, we do stand-by our Abpromise and so if you do have any problems with the antibody, then we would be happy to help out as much as possible and replace or refund the antibody if necessary. Please let me know if there is anything...

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Thank you for contacting Abcam. The antibody ab13421 was deemed not to react with mouse and rat tissue because of sequence homology. The peptide used to generate the antibody (aa21-31 of Cow Osteocalcin) has a low level of similarity to the mouse and r...

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Merci pour ces précisions. Nous sommes désolés d'apprendre que le produit ab13420 que vous avez reçu ne fonctionne pas comme attendu. Comme convenu,j'ai procédé à l'envoi d'une unité de remplaceme...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab13421. To check the status of the order please contact our Customer...

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I can confirm that proteinase K mediated antigen retrieval has already given positive results with this antibody.

1-10 of 11 Abreviews or Q&A


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