Overview

  • Product name
    Anti-Osteocalcin antibody [OCG4]
    See all Osteocalcin primary antibodies
  • Description
    Mouse monoclonal [OCG4] to Osteocalcin
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WB, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Sheep, Rabbit, Goat, Chicken, Cow, Dog, Human, Pig
    Does not react with: Mouse, Rat
  • Immunogen

    Full length protein corresponding to Cow Osteocalcin.
    Database link: P02820

  • Epitope
    Residues 21-31
  • Positive control
    • ICC/IF: SKNSH cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab13421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1 - 1/10.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 11 kDa.
IHC-Fr Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 1 - 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 16820635

Target

  • Function
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • Sequence similarities
    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    modifications
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all

Images

  • ICC/IF image of ab13421 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12421, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Immunohistochemistry of human Synovial Sarcoma staining Osteocalcin with ab13421

  • Immunohistochemistry of human Synovial Sarcoma staining Osteocalcin with ab13421

  • Immunohistochemistry of human Chondroma staining Osteocalcin with ab13421

References

This product has been referenced in:
  • Zou L  et al. Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells. Sci Rep 6:22868 (2016). IHC . Read more (PubMed: 26980556) »
  • González S  et al. A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells. Stem Cell Res 16:358-64 (2016). Read more (PubMed: 26896856) »
See all 7 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Answer

Gracias por contactarnos.

Tenemos en el catálogo, como bien indicas, tres anticuerpos contra Osteocalcina testados en humano mediante inmunocitoquímica e immunohistoquímica: ab13418, ab13420 y ab13421.

Todos nuestros productos tienen 6 meses de garantía, y en caso de no dar la tinción esperada en las aplicaciones y especies indicadas en la datasheet se os reembolsaría el importe del mismo, o se os reemplazaría el vial.

En cuanto a la forma que reconocen, estos anticuerpos reconocen tanto la forma intracelular como la secretada.

Para hacer el pedido, si lo haces desde España puedes mandarnos un mail a mailto:orders@abcam.com o llamarnos al 91 114 65 54 indicando dirección de envío y facturación, teléfono de contacto y un CIF validado para operaciones intracomunitarias. El pago puede hacerse sin ningún problema por transferencia bancaria.

Espero haber resuelto tus dudas, si tuvieras cualquier otra consulta, no dudes en contactarme de nuevo.

Read More

Answer


Nous somme vraiment désolés de ne pas avoir pu vous fournir un bon anticorps anti-osteocalcine. J'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.

Votre référence CN est *******.

Voici les manières dont vous pouvez utiliser cette référence CN:
(1) la faire valoir contre la facture originale si celle-ci n'as pas encore été payée
(2) la faire valoir contre une prochaine facture
(3) obtenir un remboursement de la somme entière si vous n'avez pas de factures actuellement en cours avec Abcam

Si vous souhaitez recevoir un remboursement au lieu d'un avoir, veuillez demander à votre service de gestion de prendre contact avec notre département comptabilité afin que nous puissions recueillir les informations nécessaires pour cette restitution. Notre service comptabilité peut être contacté par courriel à l'adresse creditcontrol@abcam.com ou par téléphone en utilisant le lien « Contactez-nous » dans le coin en haut à droite de notre site internet. Veuillez communiquer votre numéro d'avoir lorsque vous contactez notre service comptabilité.

Vous recevrez également la référence complète de la note de crédit par courriel ou par voie postale; cette référence commençant par les lettres CGB.

J'espère que cette expérience ne vous empêchera pas de commander à nouveau l'un de nos produits.

L'équipe technique reste à votre disposition pour plus de conseils.


Bonne continuation dans votre recherche.

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Question

This email has 2 sections:
A recap of the complaint
The respondent’s survey results (section-by-section)
Recap of the complaint
---------------------------------------------------------------------------------------------------------------------
Date(s) submitted: January 5, 2012
Catalog number(s): 13420
Product(s): Mouse monoclonal [OCG3] to Osteocalcin
CCEID: 3400204
Survey results
---------------------------------------------------------------------------------------------------------------------
Telephone section
Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
A. Yes
Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
A. Yes
Protocol advice section
Q. Did Abcam's Scientific Support give you protocol advice?
A.Yes
Q. How did you feel about being given protocol advice?
A.I requested protocol advice
Q. Did the product work successfully after following the advice?
A.No
Q. Why have you not tried the protocol advice? (Select all that apply.)
A.
A.
A.
A.
Free of charge replacement section
Q. Did Abcam's Scientific Support give you a free of charge replacement?
A. Yes
Q. Did the replacement product work successfully?
A.No
Credit note / refund section
Q. Did Abcam's Scientific Support give you a credit note or a refund?
A.No
Q. Would you have preferred a different outcome from receiving a credit note or refund?
A.
Overall satisfaction section
Q. How satisfied are you with how the complaint was handled?
A.Satisfied
Q. Could we have done anything to better resolve the problem?
A.
Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
A.No, I would not mind
Q. Please provide a phone number or email address where we can reach you.
A.
Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
A.

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Answer

Merci d'avoir répondu à notre questionnaire.

Je suis désolé d'apprendre que vous rencontrez toujours des problèmes avec l'anticorps anti-osteocalcin ab13421 que je vous ai envoyé en remplacement du ab13420.

J'aimeraisvous aider à trouver rapidement une seconde solution, peut-êtreen vous remboursant, vous faire un avoir, ou vous envoyer un anticorps de remplacement parmi la liste des autres anti-osteocalcin disponibles à notre catalogue.

Merci de m'indiquer la résolution qui vous conviendrait le mieux et encore toutes nos excuses pour la gène occasionnée.

Read More

Answer

Thank you for your patience.

I have received the following information about the other 3 osteocalcin antibodies I refered to in my last email:


ab13418, ab13421: These antibodies were derived from mice that were immunized with native bovine osteocalcin, thus it isassumed thatthe predominant protein used for immunization was mature bovine osteocalcin protein. Also, ab13421 binds to gamma-carboxylated osteocalcin.

ab76690: The immune region refers to the unprocessed precursor protein. There is no modification present in the immunogen sequence.

I think - based on the above information - the antibody I recommended to you in my last email (ab35078) is most likely the best one for your purposes.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your phone call.

I have checked our osteocalcin antibodies and found one where I know for sure that it was raised against the mature human protein without any modification: ab35078
https://www.abcam.com/Osteocalcin-antibody-ab35078.html
The antibody also has been tested in ELISA.

The database entry for the human protein is as follows where you can find the protein sequence as well as how the protein is processed - in case you like to doublecheck:
http://www.uniprot.org/uniprot/P02818

For the antibodies, ab13418, ab13421 and ab76690 I will need to inquire with the lab about the location of the epitope or immunogen, respectively, in respect to the mature protein.

I will let you know what I find out.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

That is correct. However, we do stand-by our Abpromise and so if you do have any problems with the antibody, then we would be happy to help out as much as possible and replace or refund the antibody if necessary. Please let me know if there is anything else I can help you with.

Read More

Answer

Thank you for contacting Abcam. The antibody ab13421 was deemed not to react with mouse and rat tissue because of sequence homology. The peptide used to generate the antibody (aa21-31 of Cow Osteocalcin) has a low level of similarity to the mouse and rat sequences. Under our Abpromise we do guarantee all of our antibodies to work as stated on the datasheet and if you have any issues with the antibodies, we would be happy to replace or refund the original cost. Please let me know if there is anything else I can help you with.

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Answer

Merci pour ces précisions. Nous sommes désolés d'apprendre que le produit ab13420 que vous avez reçu ne fonctionne pas comme attendu. Comme convenu,j'ai procédé à l'envoi d'une unité de remplacement avec la référence ab13421. Le numéro de commande de remplacement gratuit de ce produit est *******. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition. N'hésitez pas à nous contacter lors d'une prochaine occasion.

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Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab13421. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

Read More

Question
Answer

I can confirm that proteinase K mediated antigen retrieval has already given positive results with this antibody.

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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