Anti-Osteopontin antibody [53] (ab69498)
Key features and details
- Mouse monoclonal [53] to Osteopontin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Osteopontin antibody [53]
See all Osteopontin primary antibodies -
Description
Mouse monoclonal [53] to Osteopontin -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Osteopontin.
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Positive control
- WB: HeLa, MCF-7, A431, HEK-293 and Caki-2 lysates. IHC-P: Human breast cancer tissue.
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General notes
This product was changed from ascites to tissue culture supernatant on 2nd March 2020. Lot numbers higher than GR3233119 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
53 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000. Predicted molecular weight: 33 kDa.
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IHC-P |
Use a concentration of 10 µg/ml.
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Notes |
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WB
1/1000. Predicted molecular weight: 33 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
Tissue specificity
Bone. Found in plasma. -
Sequence similarities
Belongs to the osteopontin family. -
Post-translational
modificationsExtensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6696 Human
- Omim: 166490 Human
- SwissProt: P10451 Human
- Unigene: 313 Human
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Alternative names
- BNSP antibody
- Bone sialoprotein 1 antibody
- Bone sialoprotein I antibody
see all
Images
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All lanes : Anti-Osteopontin antibody [53] (ab69498) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : MCF-7 lysate
Lane 3 : A431 lysate
Lane 4 : HEK-293 lysate
Lane 5 : Caki-2 lysate
Predicted band size: 33 kDa -
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human breast cancer tissue stained withab69498 at 10 µg/ml.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human breast cancer tissue stained with ab69498 at 10 µg/ml.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human breast cancer tissue stained with ab69498 at 10 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (34)
ab69498 has been referenced in 34 publications.
- Liu Q et al. Osteopontin inhibits osteoarthritis progression via the OPN/CD44/PI3K signal axis. Genes Dis 9:128-139 (2022). PubMed: 35005113
- Wang C et al. Serum anti-SPP1 autoantibody as a potential novel biomarker in detection of esophageal squamous cell carcinoma. BMC Cancer 22:932 (2022). PubMed: 36038839
- Nilsson G et al. White matter injury but not germinal matrix hemorrhage induces elevated osteopontin expression in human preterm brains. Acta Neuropathol Commun 9:166 (2021). PubMed: 34654477
- Yang C et al. microRNA-143-3p regulates odontogenic differentiation of human dental pulp stem cells through regulation of the osteoprotegerin-RANK ligand pathway by targeting RANK. Exp Physiol 105:876-885 (2020). PubMed: 32052500
- Xin BC et al. Berberine Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells Through Activating EGFR-MAPK-Runx2 Pathways. Pathol Oncol Res 26:1677-1685 (2020). PubMed: 31598896