• Product name
  • Description
    Goat polyclonal to Osteopontin
  • Host species
  • Tested applications
    Suitable for: Blocking, ICC/IF, WB, Neutralising, Indirect ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein (Mouse) expressed in NSO cells.

  • Positive control
    • Mouse osteopontin expressed in NSO cells.



Our Abpromise guarantee covers the use of ab11503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration. Blocks osteopontin in smooth muscle cell samples.
ICC/IF 1/50.
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 65 kDa.
Neutralising Use a concentration of 1 - 3 µg/ml. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells.
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr Use at an assay dependent dilution. PubMed: 17224478


  • Function
    Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • Tissue specificity
    Bone. Found in plasma.
  • Sequence similarities
    Belongs to the osteopontin family.
  • Post-translational
    Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • BNSP antibody
    • Bone sialoprotein 1 antibody
    • BSP I antibody
    • BSPI antibody
    • Early T lymphocyte activation 1 antibody
    • ETA 1 antibody
    • ETA1 antibody
    • MGC110940 antibody
    • Nephropontin antibody
    • OPN antibody
    • Osteopontin antibody
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
    • OSTP_HUMAN antibody
    • PSEC0156 antibody
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
    • Secreted phosphoprotein 1 antibody
    • SPP 1 antibody
    • SPP-1 antibody
    • SPP1 antibody
    • SPP1/CALPHA1 fusion antibody
    • Urinary stone protein antibody
    • Uropontin antibody
    see all


This product has been referenced in:
  • Sharon Y  et al. Tumor-derived osteopontin reprograms normal mammary fibroblasts to promote inflammation and tumor growth in breast cancer. Cancer Res 75:963-73 (2015). IHC-P ; Mouse . Read more (PubMed: 25600648) »
  • Shibasaki M  et al. Pitavastatin Reduces Inflammation in Atherosclerotic Plaques in Apolipoprotein E-Deficient Mice with Late Stage Renal Disease. PLoS One 10:e0138047 (2015). Read more (PubMed: 26367531) »

See all 7 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. ab11503 Osteopontin antibody has only been tested by ELISA, Neutralisation and western blotting. That is not to say that it will not work by immunhistochemistry; just that it is yet to be tested. Searching our on lin...

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The immunogen of antibody is from amino acid 170-183 of human Osteopontin http://www.uniprot.org/uniprot/P10451. The antibody should bind to full length protein as well the immunogen sequence containing fragment.

Thank you for contacting us.
The Isotype control ab37373 can be used with ab11503.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Both of these antibodies are IgG antibodies. Unfortunately, we have not determined the subclass(es) of IgG present but I would expect there to be a mix of subclasses with possibly one predominating but this could vary depending on the immune response o...

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Vielen Dank für Ihre Anfrage.

Es tut mir ausgesprochen leid, aber wir haben leider keinen Antikörper, der für Ihre Zwecke geeignet zu sein scheint. Haben Sie es schon auf der Biocompare Seite versucht? Diese bieten auch ein bre...

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Thank you for contacting us.

I have received additional information from the lab regarding the procedure that was used.

1) How long should one incubate with the protein (for coating the wells)?
Answer: We coat the recombinant mou...

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Thank you for contacting us.

The lab sent me the following experimental details for neutralization:

Neutralization: this antibody has the ability to neutralize the bioactivity of recombinant mouse osteopontin. Immobilized recombina...

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Thank you for contacting us and thank you for submitting an Abreview for ab11503.

The testing discount codehas been activated when your Abreview was submitted. You can now use this code for ordering an Abcamprimary antibody of your choice.
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Thank you for contacting us.

I am sorry you are experiencing problems to submit an Abreview. As you are using a cell model I have just chosen the option “cell” from Sample type, so the system allows you to continue completing the ...

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Expiration date: XXXX

I am very pleased to hear you would like to accept our offer and test ab11503 in Rat species with Neutralising assays. This code will give you 1 free primary antibody before the e...

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