Product nameAnti-Osteopontin antibody
See all Osteopontin primary antibodies
DescriptionGoat polyclonal to Osteopontin
Tested applicationsSuitable for: Blocking, ICC/IF, WB, Neutralising, Indirect ELISA, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat
Recombinant full length protein (Mouse) expressed in NSO cells.
- Mouse osteopontin expressed in NSO cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: PBS, 5% Trehalose
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab11503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Blocking||Use at an assay dependent concentration. Blocks osteopontin in smooth muscle cell samples.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 65 kDa.|
|Neutralising||Use a concentration of 1 - 3 µg/ml. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells.|
|Indirect ELISA||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-Fr||Use at an assay dependent dilution. PubMed: 17224478|
FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Tissue specificityBone. Found in plasma.
Sequence similaritiesBelongs to the osteopontin family.
modificationsExtensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- BNSP antibody
- Bone sialoprotein 1 antibody
- BSP I antibody
This product has been referenced in:
- Sharon Y et al. Tumor-derived osteopontin reprograms normal mammary fibroblasts to promote inflammation and tumor growth in breast cancer. Cancer Res 75:963-73 (2015). IHC-P ; Mouse . Read more (PubMed: 25600648) »
- Shibasaki M et al. Pitavastatin Reduces Inflammation in Atherosclerotic Plaques in Apolipoprotein E-Deficient Mice with Late Stage Renal Disease. PLoS One 10:e0138047 (2015). Read more (PubMed: 26367531) »