Anti-Osteopontin antibody (ab11503)
Key features and details
- Goat polyclonal to Osteopontin
- Suitable for: Indirect ELISA, ICC/IF, Blocking, IHC-Fr, WB, Neutralising
- Reacts with: Mouse, Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Osteopontin antibody
See all Osteopontin primary antibodies -
Description
Goat polyclonal to Osteopontin -
Host species
Goat -
Tested applications
Suitable for: Indirect ELISA, ICC/IF, Blocking, IHC-Fr, WB, Neutralisingmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein (Mouse) expressed in NSO cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: PBS, 5% Trehalose -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Indirect ELISA |
Use at an assay dependent concentration.
|
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| Blocking |
Use at an assay dependent concentration.
Blocks osteopontin in smooth muscle cell samples. |
|
| IHC-Fr |
Use at an assay dependent concentration. PubMed: 17224478
|
|
| WB |
Use at an assay dependent concentration.
|
|
| Neutralising |
Use at an assay dependent concentration.
The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells. |
| Notes |
|---|
|
Indirect ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Blocking
Use at an assay dependent concentration. Blocks osteopontin in smooth muscle cell samples. |
|
IHC-Fr
Use at an assay dependent concentration. PubMed: 17224478 |
|
WB
Use at an assay dependent concentration. |
|
Neutralising
Use at an assay dependent concentration. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells. |
Target
-
Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
Tissue specificity
Bone. Found in plasma. -
Sequence similarities
Belongs to the osteopontin family. -
Post-translational
modificationsExtensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 20750 Mouse
- Entrez Gene: 25353 Rat
- SwissProt: P10923 Mouse
- SwissProt: P08721 Rat
- Unigene: 288474 Mouse
- Unigene: 8871 Rat
-
Alternative names
- BNSP antibody
- Bone sialoprotein 1 antibody
- Bone sialoprotein I antibody
see all
Protocols
Datasheets and documents
-
Datasheet download
References (10)
ab11503 has been referenced in 10 publications.
- Xin D et al. MSCs-extracellular vesicles attenuated neuroinflammation, synapse damage and microglial phagocytosis after hypoxia-ischemia injury by preventing osteopontin expression. Pharmacol Res 164:105322 (2021). PubMed: 33279596
- Giopanou I et al. Osteopontin drives KRAS-mutant lung adenocarcinoma. Carcinogenesis 41:1134-1144 (2020). PubMed: 31740923
- Hou YC et al. Tailoring of cardiovascular stent material surface by immobilizing exosomes for better pro-endothelialization function. Colloids Surf B Biointerfaces 189:110831 (2020). PubMed: 32058252
- Sharon Y et al. Tumor-derived osteopontin reprograms normal mammary fibroblasts to promote inflammation and tumor growth in breast cancer. Cancer Res 75:963-73 (2015). IHC-P ; Mouse . PubMed: 25600648
- Shibasaki M et al. Pitavastatin Reduces Inflammation in Atherosclerotic Plaques in Apolipoprotein E-Deficient Mice with Late Stage Renal Disease. PLoS One 10:e0138047 (2015). PubMed: 26367531
- Ge X et al. Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPAR?. Int J Mol Sci 16:28180-93 (2015). PubMed: 26703570
- Deangelis T et al. Growth of V-SRC-transformed cells in serum-free medium through the induction of growth factors. J Cell Physiol : (2012). PubMed: 23254450
- Redding KM et al. Transgenic mice expressing an intracellular fluorescent fusion of angiotensin II demonstrate renal thrombotic microangiopathy and elevated blood pressure. Am J Physiol Heart Circ Physiol 298:H1807-18 (2010). WB ; Mouse . PubMed: 20363893
- Yang X et al. Bone morphogenic protein 2 induces Runx2 and osteopontin expression in human aortic valve interstitial cells: role of Smad1 and extracellular signal-regulated kinase 1/2. J Thorac Cardiovasc Surg 138:1008-15 (2009). PubMed: 19664780
- Aikawa E et al. Multimodality molecular imaging identifies proteolytic and osteogenic activities in early aortic valve disease. Circulation 115:377-86 (2007). IHC-Fr ; Mouse . PubMed: 17224478
