Key features and details
- Rabbit polyclonal to Osteopontin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Osteopontin antibody
See all Osteopontin primary antibodies
DescriptionRabbit polyclonal to Osteopontin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
- Mouse kidney lysates; Rat shin bone and hippocampus tissues.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 32 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Tissue specificityBone. Found in plasma.
Sequence similaritiesBelongs to the osteopontin family.
modificationsExtensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- BNSP antibody
- Bone sialoprotein 1 antibody
- Bone sialoprotein I antibody
Anti-Osteopontin antibody (ab216402) at 1/300 dilution + mouse kidney lysates
Goat anti-rabbit IgG (H+L), HRP conjugated at 1/5000 dilution
Predicted band size: 32 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat shin bone tissue tissue labeling Osteopontin with ab216402 at 1/300 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat hippocampus tissue tissue labeling Osteopontin with ab216402 at 1/300 dilution followed by conjugation to the secondary antibody.
ab216402 has not yet been referenced specifically in any publications.