Anti-Osteopontin antibody (ab231736)
Key features and details
- Rabbit polyclonal to Osteopontin
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig, Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Osteopontin antibody
See all Osteopontin primary antibodies -
Description
Rabbit polyclonal to Osteopontin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig, Recombinant fragment
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant fragment (His-tag) corresponding to Rhesus monkey Osteopontin aa 1 to the C-terminus. N-terminal tag. Expressed in E. coli.
Database link: F6V2X3 -
Positive control
- IHC-P: Human cerebrum and kidney tissue. WB: Recombinant rhesus monkey Osteopontin protein. HeLa cell lysate. Pig kidney tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 35 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 35 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
Tissue specificity
Bone. Found in plasma. -
Sequence similarities
Belongs to the osteopontin family. -
Post-translational
modificationsExtensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6696 Human
- Entrez Gene: 397087 Pig
- Omim: 166490 Human
- SwissProt: P10451 Human
- SwissProt: P14287 Pig
- Unigene: 313 Human
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Alternative names
- BNSP antibody
- Bone sialoprotein 1 antibody
- Bone sialoprotein I antibody
see all
Images
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Formalin-fixed, paraffin-embedded human kidney tissue stained for Osteopontin with ab231736 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human cerebrum tissue stained for Osteopontin with ab231736 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-Osteopontin antibody (ab231736) at 2 µg/ml + Pig kidney tissue lysate
Predicted band size: 35 kDa -
Anti-Osteopontin antibody (ab231736) at 2 µg/ml + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 35 kDa -
Anti-Osteopontin antibody (ab231736) at 2 µg/ml + Recombinant rhesus monkey Osteopontin protein
Predicted band size: 35 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab231736 has been referenced in 1 publication.
- Goryszewska E et al. Pleiotropic role of prokineticin 1 in the porcine endometrium during pregnancy establishment and embryo implantation †. Biol Reprod 104:181-196 (2021). PubMed: 32997136