Product nameAnti-Osteopontin antibody
See all Osteopontin primary antibodies
DescriptionRabbit polyclonal to Osteopontin
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Guinea pig, Cow
- WB: Recombinant Human Osteopontin protein (ab87460), Jurkat cell lysate. IHC-P: Human kidney.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab33046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Tissue specificityBone. Found in plasma.
Sequence similaritiesBelongs to the osteopontin family.
modificationsExtensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- BNSP antibody
- Bone sialoprotein 1 antibody
- Bone sialoprotein I antibody
Anti-Osteopontin antibody (ab33046) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg
HRP conjugated anti Rabbit IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Osteopontin with ab33046 at 4µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.
This product has been referenced in:
- Liu L et al. Astragalin Promotes Osteoblastic Differentiation in MC3T3-E1 Cells and Bone Formation in vivo. Front Endocrinol (Lausanne) 10:228 (2019). Read more (PubMed: 31040823) »
- Zeng B et al. SPP1 promotes ovarian cancer progression via Integrin ß1/FAK/AKT signaling pathway. Onco Targets Ther 11:1333-1343 (2018). Read more (PubMed: 29559792) »