Recombinant Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (ab236213)
![Immunoprecipitation - Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (ab236213)](https://www.abcam.com/ps/products/236/ab236213/Images/ab236213-325061-anti-osteopontin-antibody-epr21139-316-immunoprecipitation.jpg "Immunoprecipitation - Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (ab236213)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21139-316] to Osteopontin - BSA and Azide free
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
Overview
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Product name
Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free
See all Osteopontin primary antibodies -
Description
Rabbit monoclonal [EPR21139-316] to Osteopontin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Osteopontin aa 1-300. The exact sequence is proprietary.
Database link: P10451 -
Positive control
- IHC-P: Human kidney tissue.
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General notes
Ab236213 is the carrier-free version of ab214050. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab236213 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21139-316 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab236213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 45, 60, 70, 90 kDa (predicted molecular weight: 35 kDa). |
Target
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Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
Tissue specificity
Bone. Found in plasma. -
Sequence similarities
Belongs to the osteopontin family. -
Post-translational
modificationsExtensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6696 Human
- Omim: 166490 Human
- SwissProt: P10451 Human
- Unigene: 313 Human
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Alternative names
- BNSP antibody
- Bone sialoprotein 1 antibody
- Bone sialoprotein I antibody
see all
Images
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Immunoprecipitation - Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (ab236213)
Osteopontin was immunoprecipitated from 0.35 mg of human kidney lysate with ab214050 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214050 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: Human kidney lysate 10 µg (Input).
Lane 2: ab214050 IP in human kidney lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214050 in human kidney lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
The multiple bands observed are likely due to expression of different isoforms, as well as glycosylation (PMID: 8761944; PMID: 11375993; PMID: 8535240; PMID: 12890718).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214050).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (ab236213)](https://www.abcam.com/ps/products/236/ab236213/Images/ab236213-325060-anti-osteopontin-antibody-epr21139-316-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (ab236213)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Osteopontin with ab214050 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in bile ducts of human liver (PMID: 16221502). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214050).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (ab236213)](https://www.abcam.com/ps/products/236/ab236213/Images/ab236213-311791-anti-osteopontin-antibody-epr21139-316-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (ab236213)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Osteopontin with ab214050 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in distal tubules of human kidney (PMID: 17938278), performed on a Leica Biosystems BOND® RX instrument. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214050).
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![Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (ab236213)](https://www.abcam.com/ps/products/236/ab236213/Images/ab236213-4-benefits-of-recombinant-antibodies.png)
Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (ab236213)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab236213 has not yet been referenced specifically in any publications.
