- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-OTUB1 antibody
See all OTUB1 primary antibodies -
DescriptionGoat polyclonal to OTUB1
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Host speciesGoat
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Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Cynomolgus monkey
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Immunogen
Synthetic peptide: C-
YKEYAEDDNIYQQK
corresponding to internal sequence amino acids 58-71 of human OTUB1 (NP_060140.2). -
Positive control
- Human brain (cerebellum) and, mouse and rat brain lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab82154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| WB | ||
| ELISA |
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 31 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionHydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to 'Lys-48'-linked ubiquitin.
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Tissue specificityIsoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid tissues such as tonsils, lymph nodes and spleen, as well as peripheral blood mononuclear cells.
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Sequence similaritiesBelongs to the peptidase C65 family.
Contains 1 OTU domain. -
DomainIn addition to ubiquitin-binding at the Cys-91 active site, a proximal ubiquitin-binding site is also present at Cys-23 Occupancy of the active site is needed to enable tight binding to the second site. Distinct binding sites for the ubiquitins may allow to discriminate among different isopeptide linkages (i.e. 'Lys-48'-, 'Lys-63'-linked polyubiquitin) in polyubiquitin substrates and achieve linkage-specific deubiquitination.
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Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 540984 Cow
- Entrez Gene: 55611 Human
- Entrez Gene: 107260 Mouse
- Entrez Gene: 293705 Rat
- Omim: 608337 Human
- SwissProt: Q96FW1 Human
- SwissProt: Q7TQI3 Mouse
- SwissProt: B2RYG6 Rat
see all -
Alternative names
- Deubiquitinating enzyme OTUB1 antibody
- hOTU1 antibody
- HSPC263 antibody
see all
Images
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Western blot - Anti-OTUB1 antibody (ab82154)Image courtesy of an Anonymous reviewAnti-OTUB1 antibody (ab82154) reactivity with reduced HEK293 cell lysate after transient transfection (48h) of control siRNA (lane 1-3) or siRNA targeting OTUB1 (lane 4 and 5). After SDS-PAGE, membranes were blocked in 5% milk TBS + 0.1% Tween for 1h at 25°C before incubation with ab82154 (1:1000 dilution in 5% milk TBS + 0.1% Tween) for 16h at 4ºC. Blots was then incubated with an anti-Goat HRP-conjugated secondary antibody before developing with ECL. Anti-tubulin blot included as a loading control.
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Western blot - Anti-OTUB1 antibody (ab82154)Anti-OTUB1 antibody (ab82154) at 0.5 µg/ml + mouse brain lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 1 hour
References
ab82154 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"