Anti-OTUB1 antibody (ab82154)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-OTUB1 antibody
See all OTUB1 primary antibodies -
Description
Goat polyclonal to OTUB1 -
Host species
Goat -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Cynomolgus monkey
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Immunogen
Synthetic peptide: C-
YKEYAEDDNIYQQK
corresponding to internal sequence amino acids 58-71 of human OTUB1 (NP_060140.2). -
Positive control
- WB: Human brain (cerebellum), mouse and rat brain tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab82154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 31 kDa. | |
| ELISA | Use at an assay dependent concentration. Peptide ELISA antibody detection limit dilution 1/64000. |
Target
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Function
Hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to 'Lys-48'-linked ubiquitin. -
Tissue specificity
Isoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid tissues such as tonsils, lymph nodes and spleen, as well as peripheral blood mononuclear cells. -
Sequence similarities
Belongs to the peptidase C65 family.
Contains 1 OTU domain. -
Domain
In addition to ubiquitin-binding at the Cys-91 active site, a proximal ubiquitin-binding site is also present at Cys-23 Occupancy of the active site is needed to enable tight binding to the second site. Distinct binding sites for the ubiquitins may allow to discriminate among different isopeptide linkages (i.e. 'Lys-48'-, 'Lys-63'-linked polyubiquitin) in polyubiquitin substrates and achieve linkage-specific deubiquitination. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 540984 Cow
- Entrez Gene: 55611 Human
- Entrez Gene: 107260 Mouse
- Entrez Gene: 293705 Rat
- Omim: 608337 Human
- SwissProt: Q96FW1 Human
- SwissProt: Q7TQI3 Mouse
- SwissProt: B2RYG6 Rat
see all -
Alternative names
- Deubiquitinating enzyme OTUB1 antibody
- hOTU1 antibody
- HSPC263 antibody
see all
Images
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Western blot - Anti-OTUB1 antibody (ab82154)Image courtesy of an Anonymous reviewAnti-OTUB1 antibody (ab82154) reactivity with reduced HEK293 cell lysate after transient transfection (48h) of control siRNA (lane 1-3) or siRNA targeting OTUB1 (lane 4 and 5). After SDS-PAGE, membranes were blocked in 5% milk TBS + 0.1% Tween for 1h at 25°C before incubation with ab82154 (1:1000 dilution in 5% milk TBS + 0.1% Tween) for 16h at 4ºC. Blots was then incubated with an anti-Goat HRP-conjugated secondary antibody before developing with ECL. Anti-tubulin blot included as a loading control.
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Western blot - Anti-OTUB1 antibody (ab82154)Anti-OTUB1 antibody (ab82154) at 0.5 µg/ml + mouse brain lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 1 hour
Datasheets and documents
References
ab82154 has not yet been referenced specifically in any publications.
