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    otub2-antibody-ab74371.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Deubiquitination
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Anti-OTUB2 antibody (ab74371)

  • Datasheet
Reviews (1) Submit a question References (1)

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Western blot - Anti-OTUB2 antibody (ab74371)

    Key features and details

    • Rabbit polyclonal to OTUB2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Recombinant Human OTUB2 protein (ab106896)
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    Anti-SOX2 antibody [EPR3131] (ab92494)

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    Overview

    • Product name

      Anti-OTUB2 antibody
    • Description

      Rabbit polyclonal to OTUB2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse
    • Immunogen

      Synthetic peptide derived from an internal region of human OTUB2.

    • Positive control

      • Extracts from HepG2, Jurkat, COLO 205, HT-29 and 293 cells.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

      Without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Deubiquitination
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Developmental Families
      • Other
      • Cancer
      • Signal transduction
      • Other
      • Epigenetics and Nuclear Signaling
      • Ubiquitin & Ubiquitin Like Modifiers
      • Deubiquitination

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human OTUB2 protein (ab106896)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab74371 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
    Notes
    WB
    1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

    Target

    • Function

      Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.
    • Tissue specificity

      Widely expressed. Expressed at higher level in brain.
    • Sequence similarities

      Belongs to the peptidase C65 family.
      Contains 1 OTU domain.
    • Target information above from: UniProt accession Q96DC9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 78990 Human
      • Entrez Gene: 68149 Mouse
      • Omim: 608338 Human
      • SwissProt: Q96DC9 Human
      • SwissProt: Q9CQX0 Mouse
      • Unigene: 278815 Human
      • Unigene: 127378 Mouse
      • Alternative names

        • C14orf137 antibody
        • Deubiquitinating enzyme OTUB2 antibody
        • FLJ21916 antibody
        • MGC3102 antibody
        • OTB2 antibody
        • OTU domain containing ubiquitin aldehyde binding protein 2 antibody
        • OTU domain, ubiquitin aldehyde binding 2 antibody
        • OTU domain-containing ubiquitin aldehyde-binding protein 2 antibody
        • OTU2 antibody
        • Otub2 antibody
        • OTUB2_HUMAN antibody
        • Otubain 2 antibody
        • Otubain-2 antibody
        • Ubiquitin specific processing protease OTUB2 antibody
        • Ubiquitin specific protease otubain 2 antibody
        • Ubiquitin thioesterase OTUB2 antibody
        • Ubiquitin-specific-processing protease OTUB2 antibody
        see all

      Images

      • Western blot - Anti-OTUB2 antibody (ab74371)
        Western blot - Anti-OTUB2 antibody (ab74371)
        All lanes : Anti-OTUB2 antibody (ab74371) at 1/500 dilution

        Lane 1 : Extract from HepG2 cells
        Lane 2 : Extract from Jurkat cells
        Lane 3 : Extract from COLO 205 cells
        Lane 4 : Extract from HT-29 cells
        Lane 5 : Extract from 293 cells
        Lane 6 : Extract from COLO 205 cells with immunising peptide at 5 µg

        Lysates/proteins at 5 µg per lane.

        Predicted band size: 27 kDa
        Observed band size: 27 kDa
        Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (1)

      Publishing research using ab74371? Please let us know so that we can cite the reference in this datasheet.

      ab74371 has been referenced in 1 publication.

      • Zhang S  et al. Deubiquitinase USP13 dictates MCL1 stability and sensitivity to BH3 mimetic inhibitors. Nat Commun 9:215 (2018). PubMed: 29335437

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-OTUB2 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      30 µg
      Gel Running Conditions
      Reduced Denaturing
      Sample
      Human Cell lysate - whole cell (U2OS)
      Specification
      U2OS
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Dr. Carlos le Sage

      Verified customer

      Submitted Oct 01 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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