Anti-OTUB2 antibody (ab74371)
Key features and details
- Rabbit polyclonal to OTUB2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-OTUB2 antibody -
Description
Rabbit polyclonal to OTUB2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide derived from an internal region of human OTUB2.
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Positive control
- Extracts from HepG2, Jurkat, COLO 205, HT-29 and 293 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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| Notes |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
Target
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Function
Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. -
Tissue specificity
Widely expressed. Expressed at higher level in brain. -
Sequence similarities
Belongs to the peptidase C65 family.
Contains 1 OTU domain. - Information by UniProt
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Database links
- Entrez Gene: 78990 Human
- Entrez Gene: 68149 Mouse
- Omim: 608338 Human
- SwissProt: Q96DC9 Human
- SwissProt: Q9CQX0 Mouse
- Unigene: 278815 Human
- Unigene: 127378 Mouse
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Alternative names
- C14orf137 antibody
- Deubiquitinating enzyme OTUB2 antibody
- FLJ21916 antibody
see all
Images
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Western blot - Anti-OTUB2 antibody (ab74371)All lanes : Anti-OTUB2 antibody (ab74371) at 1/500 dilution
Lane 1 : Extract from HepG2 cells
Lane 2 : Extract from Jurkat cells
Lane 3 : Extract from COLO 205 cells
Lane 4 : Extract from HT-29 cells
Lane 5 : Extract from 293 cells
Lane 6 : Extract from COLO 205 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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Datasheet download
References (1)
ab74371 has been referenced in 1 publication.
- Zhang S et al. Deubiquitinase USP13 dictates MCL1 stability and sensitivity to BH3 mimetic inhibitors. Nat Commun 9:215 (2018). PubMed: 29335437
