Key features and details
- Rabbit polyclonal to OTUB2
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-OTUB2 antibody
DescriptionRabbit polyclonal to OTUB2
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from an internal region of human OTUB2.
- Extracts from HepG2, Jurkat, COLO 205, HT-29 and 293 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).|
FunctionHydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.
Tissue specificityWidely expressed. Expressed at higher level in brain.
Sequence similaritiesBelongs to the peptidase C65 family.
Contains 1 OTU domain.
- Information by UniProt
- C14orf137 antibody
- Deubiquitinating enzyme OTUB2 antibody
- FLJ21916 antibody
All lanes : Anti-OTUB2 antibody (ab74371) at 1/500 dilution
Lane 1 : Extract from HepG2 cells
Lane 2 : Extract from Jurkat cells
Lane 3 : Extract from COLO 205 cells
Lane 4 : Extract from HT-29 cells
Lane 5 : Extract from 293 cells
Lane 6 : Extract from COLO 205 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
ab74371 has been referenced in 1 publication.
- Zhang S et al. Deubiquitinase USP13 dictates MCL1 stability and sensitivity to BH3 mimetic inhibitors. Nat Commun 9:215 (2018). PubMed: 29335437