Anti-OTUB2 antibody (ab74371)

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Overview

  • Product name

    Anti-OTUB2 antibody

  • Description

    Rabbit polyclonal to OTUB2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide derived from an internal region of human OTUB2.

  • Positive control

    • Extracts from HepG2, Jurkat, COLO 205, HT-29 and 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ELISA 1/20000.

Target

  • Function

    Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.

  • Tissue specificity

    Widely expressed. Expressed at higher level in brain.

  • Sequence similarities

    Belongs to the peptidase C65 family.
    Contains 1 OTU domain.
  • Information by UniProt

  • Database links

    • Alternative names

      • C14orf137 antibody
      • Deubiquitinating enzyme OTUB2 antibody
      • FLJ21916 antibody
      • MGC3102 antibody
      • OTB2 antibody
      • OTU domain containing ubiquitin aldehyde binding protein 2 antibody
      • OTU domain, ubiquitin aldehyde binding 2 antibody
      • OTU domain-containing ubiquitin aldehyde-binding protein 2 antibody
      • OTU2 antibody
      • Otub2 antibody
      • OTUB2_HUMAN antibody
      • Otubain 2 antibody
      • Otubain-2 antibody
      • Ubiquitin specific processing protease OTUB2 antibody
      • Ubiquitin specific protease otubain 2 antibody
      • Ubiquitin thioesterase OTUB2 antibody
      • Ubiquitin-specific-processing protease OTUB2 antibody
      see all

    Images

    • Western blot - Anti-OTUB2 antibody (ab74371)
      Western blot - Anti-OTUB2 antibody (ab74371)
      All lanes : Anti-OTUB2 antibody (ab74371) at 1/500 dilution

      Lane 1 : Extract from HepG2 cells
      Lane 2 : Extract from Jurkat cells
      Lane 3 : Extract from COLO 205 cells
      Lane 4 : Extract from HT-29 cells
      Lane 5 : Extract from 293 cells
      Lane 6 : Extract from COLO 205 cells with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 27 kDa
      Observed band size: 27 kDa
      Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Customer reviews and Q&As

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    Western blot abreview for Anti-OTUB2 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (U2OS)
    Specification
    U2OS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Carlos le Sage

    Verified customer

    Submitted Oct 01 2013

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