Overview

  • Product name

    Anti-OTUD4/HIN-1 antibody
  • Description

    Rabbit polyclonal to OTUD4/HIN-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human OTUD4/HIN-1 (C terminal).
    Database link: NP_001096123
    (Peptide available as ab217680)

  • Positive control

    • Daudi cell lysate, Daudi cells.
  • General notes

     This product was previously labelled as OTUD4

     

Properties

Applications

Our Abpromise guarantee covers the use of ab106368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 124 kDa.
ICC Use a concentration of 2.5 µg/ml.

Target

Images

  • Lane 1 : Anti-OTUD4/HIN-1 antibody (ab106368) at 0.25 µg/ml
    Lane 2 : Anti-OTUD4/HIN-1 antibody (ab106368) at 0.5 µg/ml

    All lanes : Daudi (human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 124 kDa

  • ab106368, at 2.5 ug/ml, staining OTUD4/HIN-1 in Daudi cells by immunocytochemistry.

  • Immunofluorescence of OTUD4/HIN-1 in Daudi cells using ab106368 at 20 ug/ml.

References

This product has been referenced in:

  • Esquerdo M  et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS One 11:e0150364 (2016). IHC . Read more (PubMed: 26934049) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (lymphoid)
Specification
lymphoid
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 04 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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