Anti-DUBA antibody (ab176727)
Key features and details
- Rabbit polyclonal to DUBA
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DUBA antibody
See all DUBA primary antibodies -
Description
Rabbit polyclonal to DUBA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human DUBA aa 150-500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_060072.1
Database link: Q96G74 -
Positive control
- HeLa, 293T and NIH3T3 cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 61 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 61 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Negative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. -
Tissue specificity
Expressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes. -
Sequence similarities
Belongs to the peptidase C85 family.
Contains 1 OTU domain. - Information by UniProt
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Database links
- Entrez Gene: 55593 Human
- Entrez Gene: 54644 Mouse
- Entrez Gene: 363452 Rat
- Omim: 300713 Human
- SwissProt: Q96G74 Human
- SwissProt: Q3U2S4 Mouse
- SwissProt: Q2YDU3 Rat
- Unigene: 496098 Human
see all -
Alternative names
- Deubiquinating enzyme A antibody
- Deubiquitinating enzyme A antibody
- DKFZp761A052 antibody
see all
Images
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Western blot of immunoprecipitate of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), labeling DUBA using ab176727 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate, using chemiluminescence with exposure times of 10 seconds.
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All lanes : Anti-DUBA antibody (ab176727) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 61 kDaChemiluminescence with exposure times of 3 minutes.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab176727 has been referenced in 2 publications.
- Cho JH et al. Deubiquitinase OTUD5 is a positive regulator of mTORC1 and mTORC2 signaling pathways. Cell Death Differ 28:900-914 (2021). PubMed: 33110214
- Cho JH et al. The p90 ribosomal S6 kinase-UBR5 pathway controls Toll-like receptor signaling via miRNA-induced translational inhibition of tumor necrosis factor receptor-associated factor 3. J Biol Chem 292:11804-11814 (2017). PubMed: 28559278