Overview

  • Product name
  • Description
    Rabbit polyclonal to OTUD5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human OTUD5 aa 150-500. The exact sequence is proprietary. NP_060072.1
    Database link: Q96G74

  • Positive control
    • HeLa, 293T and NIH3T3 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab176727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 61 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Negative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production.
  • Tissue specificity
    Expressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the peptidase C85 family.
    Contains 1 OTU domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquinating enzyme A antibody
    • Deubiquitinating enzyme A antibody
    • DKFZp761A052 antibody
    • DUBA antibody
    • MGC104871 antibody
    • OTTHUMP00000025821 antibody
    • OTU domain containing 5 antibody
    • OTU domain containing protein 5 antibody
    • OTU domain-containing protein 5 antibody
    • OTUD 5 antibody
    • otud5 antibody
    • OTUD5_HUMAN antibody
    see all

Images

  • Western blot of immunoprecipitate of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), labeling OTUD5 using ab176727 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate, using chemiluminescence with exposure times of 10 seconds.

  • All lanes : Anti-OTUD5 antibody (ab176727) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 61 kDa



    Chemiluminescence with exposure times of 3 minutes.

References

ab176727 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab176727.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up