Anti-DUBA antibody (ab225558)
Key features and details
- Rabbit polyclonal to DUBA
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DUBA antibody
See all DUBA primary antibodies -
Description
Rabbit polyclonal to DUBA -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide within Human DUBA aa 521-571. The exact sequence is proprietary. (NP_060072.1).
Database link: Q96G74 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab225558 was affinity purified using an epitope specific to DUBA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-5 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 61 kDa.
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Notes |
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IP
Use at 2-5 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 61 kDa. |
Target
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Function
Negative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. -
Tissue specificity
Expressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes. -
Sequence similarities
Belongs to the peptidase C85 family.
Contains 1 OTU domain. - Information by UniProt
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Database links
- Entrez Gene: 737927 Chimpanzee
- Entrez Gene: 539145 Cow
- Entrez Gene: 101925979 Cynomolgus monkey
- Entrez Gene: 480911 Dog
- Entrez Gene: 100062519 Horse
- Entrez Gene: 55593 Human
- Entrez Gene: 54644 Mouse
- Entrez Gene: 100460247 Orangutan
see all -
Alternative names
- Deubiquinating enzyme A antibody
- Deubiquitinating enzyme A antibody
- DKFZp761A052 antibody
see all
Images
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All lanes : Anti-DUBA antibody (ab225558) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 30 seconds -
DUBA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225558 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225558 at 1 µg/ml.
Lane 1: ab225558 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab225558 has not yet been referenced specifically in any publications.