Anti-OTULIN antibody (ab114137)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-OTULIN antibody
See all OTULIN primary antibodies -
Description
Rabbit polyclonal to OTULIN -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Chimpanzee, Orangutan
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Immunogen
Synthetic peptide corresponding to Human OTULIN aa 1-100 conjugated to keyhole limpet haemocyanin.
Database link: Q96BN8 -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; U2OS
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab114137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
Target
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Sequence similarities
Belongs to the FAM105 family. - Information by UniProt
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Database links
- Entrez Gene: 90268 Human
- Entrez Gene: 432940 Mouse
- SwissProt: Q96BN8 Human
- SwissProt: Q3UCV8 Mouse
- Unigene: 406335 Human
- Unigene: 309164 Mouse
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Alternative names
- AIPDS antibody
- Deubiquitinating enzyme otulin antibody
- F105B_HUMAN antibody
see all
Images
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Western blot - Anti-OTULIN antibody (ab114137)All lanes : Anti-OTULIN antibody (ab114137) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 73 kDa (possible non-specific binding)
Exposure time: 12 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab114137 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
References
ab114137 has not yet been referenced specifically in any publications.
