Product nameAnti-OTULIN antibody
See all OTULIN primary antibodies
DescriptionRabbit polyclonal to OTULIN
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human kidney tissue; RT 4, U 251 MG and Human liver lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500. Predicted molecular weight: 40 kDa.|
Sequence similaritiesBelongs to the FAM105 family.
- Information by UniProt
- AIPDS antibody
- Deubiquitinating enzyme otulin antibody
- F105B_HUMAN antibody
All lanes : Anti-OTULIN antibody (ab151117) at 1/100 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 40 kDa
Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and mitochondria. Recommended concentration of ab151117 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling FAM105B with ab151117 at 1/50 dilution.
This product has been referenced in:
- Lafont E et al. The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death. EMBO J 36:1147-1166 (2017). WB ; Human . Read more (PubMed: 28258062) »
- Kupka S et al. SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes. Cell Rep 16:2271-80 (2016). WB . Read more (PubMed: 27545878) »