Recombinant Anti-OTULIN antibody [EPR19841] (ab211328)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19841] to OTULIN
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-OTULIN antibody [EPR19841]
See all OTULIN primary antibodies -
Description
Rabbit monoclonal [EPR19841] to OTULIN -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T, MCF7 and PC-3 cell lysates; Human brain, fetal heart and fetal spleen lysates. IP: HEK-293T whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19841 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
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IP |
1/30.
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Notes |
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WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
IP
1/30. |
Target
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Sequence similarities
Belongs to the FAM105 family. - Information by UniProt
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Database links
- Entrez Gene: 90268 Human
- SwissProt: Q96BN8 Human
- Unigene: 406335 Human
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Alternative names
- AIPDS antibody
- Deubiquitinating enzyme otulin antibody
- F105B_HUMAN antibody
see all
Images
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All lanes : Anti-OTULIN antibody [EPR19841] (ab211328) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : OTULIN knockout HEK-293T whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 2 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-OTULIN antibody [EPR19841] (ab211328) at 1/1000 dilution
Lane 1 : Human brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal spleen lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 4-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 40 kDaExposure time : Lane 1-3: 3 minutes; Lane 4-5: 3 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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OTULIN was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate with ab211328 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211328 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: HEK-293T whole cell lysate 10 µg (Input).
Lane 2: ab211328 IP in HEK-293T whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211328 in HEK-293T whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab211328 has been referenced in 1 publication.
- Zhai D et al. QKI degradation in macrophage by RNF6 protects mice from MRSA infection via enhancing PI3K p110β dependent autophagy. Cell Biosci 12:154 (2022). PubMed: 36088389