Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Otx1 + Otx2 antibody - ChIP Grade

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (E6.0 embryo in decidua)
Specification
E6.0 embryo in decidua
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
Yes - tween
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Other product details

Dilution
1/2500
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% serum, 1% ovalbumin in PBSTween

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
After using a biotinylated secondary, ABC and then DAB was used for color development.

Dr. Jesse Mager

Verified customer

Submitted Dec 22 2008

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