Immunohistochemistry (Frozen sections) abreview for Anti-Otx1 + Otx2 antibody - ChIP Grade

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Fish Tissue sections (Medaka and zebrafish eye and brain)
Specification
Medaka and zebrafish eye and brain
Fixative
Formaldehyde
Permeabilization
Yes - Triton x-100 0.2%
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Concentration
2 µg/ml
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS + 0.2% triton + 5% Fetal calf serum

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

PubMed ID
20534668: View

Dr. Raquel Marco Ferreres

Verified customer

Submitted Jan 31 2011

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