I see specific nuclear staining in the brain of E8.5 embryos using this antibody. But I have given the antibody 3 stars, because there is a lot of unspecific membrane staining in the posterior of E8.5 sections, which I have difficulty to get rid of. I have tried to use glycine or a commercial primary dilution buffer to reduce the unspecific staining, but neither worked.
Abcam user community
Submitted Nov 05 2013
Get resources and offers direct to your inboxSign up