Western blot abreview for Anti-Otx1 + Otx2 antibody - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - nuclear (GnRH neurons)
Loading amount
20 µg
Specification
GnRH neurons
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2

Other product details

Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 40 kDa Non-specific: 28, 60, 100 kDa

Additional data

Additional Notes
Have not tried for IHC yet but plan to
Negative control is GN11 nuclear cell lysate
Positive control is GT1-7 nuclear cell lysate

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Submitted Jun 01 2006

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