Immunohistochemistry (Frozen sections) abreview for Anti-Otx1 + Otx2 antibody - ChIP Grade

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E12.5 Whole brain section)
Specification
E12.5 Whole brain section
Fixative
Paraformaldehyde
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Other product details

Dilution
1/100
Incubation time
14 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: alexa fluor 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Additional data

Additional Notes
Strong detection in the developing choroid plexus / hippocampus. PMID: 15917450. Citric acid was used in heat-mediated antigen retrieval step.
Abcam response
This antibody successfully stained mouse E12.5 whole brain sections in IHC-Fr.

Dr. Dean Griffiths

Verified customer

Submitted Jan 04 2007

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