Key features and details
- Rabbit polyclonal to Otx2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Otx2 antibody
See all Otx2 primary antibodies
DescriptionRabbit polyclonal to Otx2
SpecificityReacts specifically with 31 kDa Otx2 protein.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Synthetic peptide derived from the N terminal domain of human Otx2
- This antibody gave a positive siganl in OTX2 Overexpression Lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab76748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/100 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).|
FunctionProbably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
Tissue specificityExpressed in brain.
Involvement in diseaseDefects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5) [MIM:610125]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Up to 80% of cases of microphthalia occur in association with syndromes that include non-ocular abnormalities. MCOPS5 patients manifest unilateral or bilateral microphthalmia/clinical anophthalmia and variable additional features including coloboma, microcornea, cataract, retinal dystrophy, hypoplasia or agenesis of the optic nerve, agenesis of the corpus callosum, developmental delay, joint laxity, hypotonia, and seizures.
Sequence similaritiesBelongs to the paired homeobox family. Bicoid subfamily.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- CPHD6 antibody
- Homeobox protein OTX2 antibody
- MCOPS 5 antibody
All lanes : Anti-Otx2 antibody (ab76748) at 1 µg/ml
Lane 1 : OTX1 Recombinant Protein
at 0.1 µg
Lane 2 : OTX2 Overexpression Lysate at 20 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
Otx1 Recombinant Protein was used as a negative control. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab76748 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab76748 has been referenced in 1 publication.
- Japp AS et al. High-resolution genomic analysis does not qualify atypical plexus papilloma as a separate entity among choroid plexus tumors. J Neuropathol Exp Neurol 74:110-20 (2015). PubMed: 25575132